Publication detail

Sensing nanoparticle-protein corona using nanoparticle enhanced Laser Induced Breakdown Spectroscopy signal enhancement

DELL'AGLIO, M. SALAJKOVÁ, Z. MALLARDI, A. SPORTELLI, M.KAISER, J. CIOFFI, N. DE GIACOMO, A.

Original Title

Sensing nanoparticle-protein corona using nanoparticle enhanced Laser Induced Breakdown Spectroscopy signal enhancement

Type

journal article in Web of Science

Language

English

Original Abstract

Recently nanoparticle enhanced Laser Induced Breakdown Spectroscopy (NELIBS) is getting a growing interest as an effective alternative method for improving the analytical performance of LIBS. On the other hand, the plasmonic effect during laser ablation can be used for a different task rather than elemental analysis. In this paper, the dependence of NELIBS emission signal enhancement on nanoparticle-protein solutions dried on a reference substrate (metallic titanium) was investigated. Two proteins were studied: Human Serum Albumin (HSA) and Cytochrome C (CytC). Both proteins have a strong affinity for the gold nanoparticles (AuNPs) due to the bonding between the single free exterior thiol (associated with a cysteine residue) and the gold surface to form a stable protein corona. Then, since the protein sizes are vastly different, a different number of protein units is needed to cover AuNP surface to form a protein layer. The NP-protein solution was dropped and dried onto the titanium substrate. Then the NELIBS signal enhancement of Ti emission lines was correlated to the solution characteristics as determined with Dynamic Light Scattering (DLS), Surface Plasmon Resonance (SPR) spectroscopy and Laser Doppler Electrophoresis (LDE) for zeta-potential determination. Moreover, the dried solutions were studied with TEM (Transmission Electron Microscopy) for the inspection of the inter-particle distance. The structural effect of the NP-protein conjugates on the NELIBS signal reveals that NELIBS can be used to determine the number of protein units required to form the nanoparticle-protein corona with good accuracy. Although the investigated NP-protein systems are simple cases in biological applications, this work demonstrates, for the first time, a different use of NELIBS that is beyond elemental analysis and it opens the way for sensing the nanoparticle protein corona.

Keywords

NELIBS; Gold nanoparticles; Nanoparticle protein corona; Human serum albumin; Cytochrome C; Naked AuNPs by PLAL

Authors

DELL'AGLIO, M.; SALAJKOVÁ, Z.; MALLARDI, A.; SPORTELLI, M.;KAISER, J.; CIOFFI, N.; DE GIACOMO, A.

Released

23. 7. 2021

Publisher

ELSEVIER

Location

AMSTERDAM

ISBN

0039-9140

Periodical

Talanta

Year of study

235

Number

122741

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

8

Pages count

8

URL

https://www.sciencedirect.com/science/article/pii/S0039914021006627

BibTex

@article{BUT173072,
  author="DELL'AGLIO, M. and SALAJKOVÁ, Z. and MALLARDI, A. and SPORTELLI, M. and KAISER, J. and CIOFFI, N. and DE GIACOMO, A.",
  title="Sensing nanoparticle-protein corona using nanoparticle enhanced Laser Induced Breakdown Spectroscopy signal enhancement",
  journal="Talanta",
  year="2021",
  volume="235",
  number="122741",
  pages="1--8",
  doi="10.1016/j.talanta.2021.122741",
  issn="0039-9140",
  url="https://www.sciencedirect.com/science/article/pii/S0039914021006627"
}