Publication detail

Upgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heater

SKOPALÍK, J. SEKORA, J. PEŠL, M. BÉBAROVÁ, M. ŠVECOVÁ, O. PARÁK, T. ČMIEL, V. PROVAZNÍK, I. JEKLOVÁ, E. MAŠEK, J.

Original Title

Upgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heater

Type

journal article in Scopus

Language

English

Original Abstract

Biological experiments involving isolated organs and tissues demand precise temperature monitoring and regulation. An automatic temperature control system was proposed and optimised on real isolated swine hearts and the prototype is described in this work. The traditional Langendorff apparatus consists of a heart holder, a reservoir of perfusion solution flowing to aortic cannula and a heating bath allowing passive heat transfer to the reservoir of perfusion solution. The commercial infrared camera FLIR T62101 was added to this basic set-up and used for very precise monitoring of the temperature kinetic of the organ and connected with an electronic feedback loop, which allowed real-time and precise regulation of heat transfer from the heating bath to the perfusion solution and in turn indirectly to the heart tissue. This provides real time control and active regulation of the myocardial tissue temperature. The infrared camera was tested in several modes and several variants of detection were optimised for ideal measurement of the region of interest of the ex vivo organ. The kinetics of the temperature changes and temperature stability of the tissue were recorded and calibrated by external electronic thermometers (type Pt100, inserted in tissue). The time lapse from the hang-up of the hypo termed organ (30 °C) until optimal warming (37 °C) was less than eight minutes in the final instrument prototype. The final stability of the 37 °C tissue temperature was approved; the temperature fluctuation of left ventricle tissue was characterised as 36.8 ± 0.5 °C. This upgraded traditional instrument could be used in specific preclinical and clinical transplantation and analytical projects in future.

Keywords

isolated heart; heart perfusion; tissue thermostability; thermo controller; IR thermometry

Authors

SKOPALÍK, J.; SEKORA, J.; PEŠL, M.; BÉBAROVÁ, M.; ŠVECOVÁ, O.; PARÁK, T.; ČMIEL, V.; PROVAZNÍK, I.; JEKLOVÁ, E.; MAŠEK, J.

Released

1. 3. 2021

Publisher

Czech Society for Biomedical Engineering and Medical Informatics

Location

Prague, Czech Republic

ISBN

0301-5491

Periodical

Lékař a technika

Year of study

50

Number

4

State

Czech Republic

Pages from

137

Pages to

141

Pages count

5

URL

https://ojs.cvut.cz/ojs/index.php/CTJ/article/view/7126

Full text in the Digital Library

http://hdl.handle.net/11012/203210

BibTex

@article{BUT173201,
  author="Josef {Skopalík} and Jiří {Sekora} and Martin {Pešl} and Markéta {Bébarová} and Olga {Švecová} and Tomáš {Parák} and Vratislav {Čmiel} and Valentine {Provazník} and Edita {Jeklová} and Josef {Mašek}",
  title="Upgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heater",
  journal="Lékař a technika",
  year="2021",
  volume="50",
  number="4",
  pages="137--141",
  doi="10.14311/CTJ.2020.4.03",
  issn="0301-5491",
  url="https://ojs.cvut.cz/ojs/index.php/CTJ/article/view/7126"
}