Publication detail

Parametric Deconvolution for Cancer Cells Viscoelasticity Measurements from Quantitative Phase Images

VIČAR, T. GUMULEC, J. KOLÁŘ, R. CHMELÍK, J. NAVRATIL, J. CHMELÍKOVÁ, L. ČMIEL, V. PROVAZNÍK, I. MASAŘÍK, M.

Original Title

Parametric Deconvolution for Cancer Cells Viscoelasticity Measurements from Quantitative Phase Images

Type

conference paper

Language

English

Original Abstract

In this contribution, we focused on optimising a dynamic flow-based shear stress system to achieve a reliable platform for cell shear modulus (stiffness) and viscosity assessment using quantitative phase imaging. The estimation of cell viscoelastic properties is influenced by distortion of the shear stress waveform, which is caused by the properties of the flow system components (i.e., syringe, flow chamber and tubing). We observed that these components have a significant influence on the measured cell viscoelastic characteristics. To suppress this effect, we applied a correction method utilizing parametric deconvolution of the flow system's optimized impulse response. Achieved results were compared with the direct fitting of the Kelvin-Voigt viscoelastic model and the basic steady-state model. The results showed that our novel parametric deconvolution approach is more robust and provides a more reliable estimation of viscosity with respect to changes in the syringe's compliance compared to Kelvin-Voigt model.

Keywords

shear stress;quantitative phase imaging;viscoelastic model

Authors

VIČAR, T.; GUMULEC, J.; KOLÁŘ, R.; CHMELÍK, J.; NAVRATIL, J.; CHMELÍKOVÁ, L.; ČMIEL, V.; PROVAZNÍK, I.; MASAŘÍK, M.

Released

18. 11. 2021

Publisher

IEEE

Location

345 E 47TH ST, NEW YORK, NY 10017 USA

ISBN

978-1-7281-1179-7

Book

IEEE Engineering in Medicine and Biology Society Conference Proceedings

ISBN

1557-170X

Periodical

Proceedings IEEE EMBC

State

United States of America

Pages from

439

Pages to

442

Pages count

4

URL

https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=9630524

BibTex

@inproceedings{BUT173255,
  author="Tomáš {Vičar} and Jaromír {Gumulec} and Radim {Kolář} and Jiří {Chmelík} and Jiri {Navratil} and Larisa {Chmelíková} and Vratislav {Čmiel} and Valentine {Provazník} and Michal {Masařík}",
  title="Parametric Deconvolution for Cancer Cells Viscoelasticity Measurements from Quantitative Phase Images",
  booktitle="IEEE Engineering in Medicine and Biology Society Conference Proceedings",
  year="2021",
  journal="Proceedings IEEE EMBC",
  pages="439--442",
  publisher="IEEE",
  address="345 E 47TH ST, NEW YORK, NY 10017 USA",
  doi="10.1109/EMBC46164.2021.9630524",
  isbn="978-1-7281-1179-7",
  issn="1557-170X",
  url="https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=9630524"
}