Publication detail

Increasing the Image Contrast via Fast Fluorescence Photobleaching

KOLÁŘ, R. VIČAR, T. CHMELÍKOVÁ, L. PROVAZNÍK, I.

Original Title

Increasing the Image Contrast via Fast Fluorescence Photobleaching

Type

conference paper

Language

English

Original Abstract

This paper focuses on the analysis of image sequences acquired during fast photobleaching using a standard wide-field microscope. We show that the photobleaching rate estimated for each pixel is not constant for the whole field of view, but it provides a new spatially variant parametric image related to the cell structure and diffusion of fluorophores. We also provide an alternative way to estimate a pixel-wise photobleaching rate with significantly less computation time than exponential model fitting.

Keywords

image sequences;photobleaching;microscopy

Authors

KOLÁŘ, R.; VIČAR, T.; CHMELÍKOVÁ, L.; PROVAZNÍK, I.

Released

18. 11. 2021

Publisher

Elsevier

ISBN

978-1-7281-1179-7

Book

2021 43nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)

Pages from

2741

Pages to

2744

Pages count

4

URL

https://ieeexplore.ieee.org/document/9630859

BibTex

@inproceedings{BUT173256,
  author="Radim {Kolář} and Tomáš {Vičar} and Larisa {Chmelíková} and Valentine {Provazník}",
  title="Increasing the Image Contrast via Fast Fluorescence Photobleaching",
  booktitle="2021 43nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)",
  year="2021",
  pages="2741--2744",
  publisher="Elsevier",
  doi="10.1109/EMBC46164.2021.9630859",
  isbn="978-1-7281-1179-7",
  url="https://ieeexplore.ieee.org/document/9630859"
}