Publication detail

Computational image enhancement of multimode fibre-based holographic endo-microscopy: harnessing the muddy modes

TUČKOVÁ, T. ŠILER, M. FLAES, D. JÁKL, P. TURTAEV, S. KRÁTKÝ, S. HEINTZMANN, R. UHLÍŘOVÁ, H. ČIŽMÁR, T.

Original Title

Computational image enhancement of multimode fibre-based holographic endo-microscopy: harnessing the muddy modes

Type

journal article in Web of Science

Language

English

Original Abstract

In imaging geometries, which employ wavefront-shaping to control the light transport through a multi-mode optical fibre (MMF), this terminal hair-thin optical component acts as a minimally invasive objective lens, enabling high resolution laser-scanning fluorescence microscopy inside living tissues at depths hardly accessible by any other light-based technique. Even in the most advanced systems, the diffraction-limited foci scanning the object across the focal plane are contaminated by a stray optical signal carrying typically few tens of % of the total optical power. The stray illumination takes the shape of a randomised but reproducible speckle, and is unique for each position of the focus. We experimentally demonstrate that the performance of imaging a fluorescent object can be significantly improved, when resulting images are computationally post-processed, utilising records of intensities of all speckle-contaminated foci used in the imaging procedure. We present two algorithms based on a regularised iterative inversion and regularised direct pseudo-inversion respectively which lead to enhancement of the image contrast and resolution. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Keywords

LIGHT; TRANSFORMATION; TRANSMISSION; RESOLUTION; PHASE

Authors

TUČKOVÁ, T.; ŠILER, M.; FLAES, D.; JÁKL, P.; TURTAEV, S.; KRÁTKÝ, S.; HEINTZMANN, R.; UHLÍŘOVÁ, H.; ČIŽMÁR, T.

Released

8. 11. 2021

Publisher

Optica Publishing Group

Location

WASHINGTON

ISBN

1094-4087

Periodical

OPTICS EXPRESS

Year of study

29

Number

23

State

United States of America

Pages from

38206

Pages to

38220

Pages count

15

URL

https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-29-23-38206&id=464465

Full text in the Digital Library

http://hdl.handle.net/11012/203100

BibTex

@article{BUT174094,
  author="Tereza {Chmelíková} and Martin {Šiler} and Dirk E. Boonzajer {Flaes} and Petr {Jákl} and Sergey {Turtaev} and Stanislav {Krátký} and Rainer {Heintzmann} and Hana {Uhlířová} and Tomáš {Čižmár}",
  title="Computational image enhancement of multimode fibre-based holographic endo-microscopy: harnessing the muddy modes",
  journal="OPTICS EXPRESS",
  year="2021",
  volume="29",
  number="23",
  pages="38206--38220",
  doi="10.1364/OE.434848",
  issn="1094-4087",
  url="https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-29-23-38206&id=464465"
}