Publication detail

Self encapsulation of plant growth-promoting bacteria Azotobacter vinelandii for their use as next-generation bioinoculants

ČERNAYOVÁ, D. SÚKENÍK, M. SEDLÁČEK, P. OBRUČA, S.

Original Title

Self encapsulation of plant growth-promoting bacteria Azotobacter vinelandii for their use as next-generation bioinoculants

Type

abstract

Language

English

Original Abstract

Azotobacter vinelandii is a plant-growth-promoting bacterium (PGPB), capable of synthesizing two different biopolymers with great application potential. Polyhydroxyalkanoates (PHA) are produced and stored in a form of intracellular granules, whereas alginate is produced extracellularly. Plant growth-promoting bacteria can be used as bio-inoculants to increase yields in agriculture. Encapsulation of bacteria into hydrogel-based carriers substantially improves the application potential of the bioinoculant as well as the viability and robustness of the bacterial culture. In this work, the alginate produced by the bacteria themselves was crosslinked with a calcium chloride solution to form a hydrogel carrier with encapsulated bacteria. This unconventional concept that we ter- med „self encapsulation“ simplifies bacterial encapsulation and production of hydrogel carriers, reduces the cost, and expands its usability in numerous applications. Firstly, we screened five strains of Azotobacter vinelandii to select those with a sufficient production capacity concerning alginate and PHA and suitable gelationproperties. Actually, the strain DSM 87 reached the highest concentration of the alginate (4.9 ± 0.6 g/l). The alginates produced by strains DSM 87 and DSM 720 demonstrated the best gelation properties. The bacterial entrapped in a gel matrix seemed to beviable and cultivatable. Hence, the preliminary results confirmed the feasibility and viability of the novel concept.

Keywords

Azotobacter vinelandii, polyhydroxyalkanoates, alginate, gels, encapsulation

Authors

ČERNAYOVÁ, D.; SÚKENÍK, M.; SEDLÁČEK, P.; OBRUČA, S.

Released

25. 11. 2021

Publisher

Vysoké učení technické v Brně, Fakulta chemická

Location

Brno

ISBN

978-80-214-6002-7

Book

CHEMIE JE ŽIVOT 2021

Edition

1.

Edition number

1

Pages from

32

Pages to

33

Pages count

2

URL

https://www.fch.vut.cz/vav/konference/sok/vystupy/sbornik-konference-2021-abstrakty-a5-pdf-p213391

BibTex

@misc{BUT175308,
  author="Diana {Černayová} and Martin {Súkeník} and Petr {Sedláček} and Stanislav {Obruča}",
  title="Self encapsulation of plant growth-promoting bacteria Azotobacter vinelandii for their use as next-generation bioinoculants",
  booktitle="CHEMIE JE ŽIVOT 2021",
  year="2021",
  series="1.",
  edition="1",
  pages="32--33",
  publisher="Vysoké učení technické v Brně, Fakulta chemická",
  address="Brno",
  isbn="978-80-214-6002-7",
  url="https://www.fch.vut.cz/vav/konference/sok/vystupy/sbornik-konference-2021-abstrakty-a5-pdf-p213391",
  note="abstract"
}