Publication detail

Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization

DUFKOVÁ, K. BEZDÍČEK, M. ČUPROVÁ, K. PANTŮČKOVÁ, D. NYKRÝNOVÁ, M. BRHELOVÁ, E. KOCMANOVÁ, I. HODOVÁ, S. HANSLIANOVA, M. JUREN, T. LIPOVÝ, B. MAYER, J. LENGEROVÁ, M.

Original Title

Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization

Type

journal article in Web of Science

Language

English

Original Abstract

Staphylococcus aureus is a major bacterial human pathogen that causes a wide variety of clinical manifestations. The main aim of the presented study was to determine and optimize a novel sequencing independent approach that enables molecular typing of S. aureus isolates and elucidates the transmission of emergent clones between patients. In total, 987 S. aureus isolates including both methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) isolates were used to evaluate the novel typing approach combining high-resolution melting (HRM) analysis of multilocus sequence typing (MLST) genes (mini-MLST) and spa gene (spa-HRM). The novel approach’s discriminatory ability was evaluated by whole-genome sequencing (WGS). The clonal relatedness of tested isolates was set by the BURP and BURST approach using spa and MLST data, respectively. Mini-MLST classified the S. aureus isolates into 38 clusters, followed by spa-HRM classifying the isolates into 101 clusters. The WGS proved HRM-based methods to effectively differentiate between related S. aureus isolates. Visualizing evolutionary relationships among different spa-types provided by the BURP algorithm showed comparable results to MLST/mini-MLST clonal clusters. We proved that the combination of mini-MLST and spa-HRM is rapid, reproducible, and cost-efficient. In addition to high discriminatory ability, the correlation between spa evolutionary relationships and mini-MLST clustering allows the variability in population structure to be monitored.

Keywords

MLST; MRSA; MSSA; high-resolution melting; mini-MLST; spa-typing; whole-genome sequencing

Authors

DUFKOVÁ, K.; BEZDÍČEK, M.; ČUPROVÁ, K.; PANTŮČKOVÁ, D.; NYKRÝNOVÁ, M.; BRHELOVÁ, E.; KOCMANOVÁ, I.; HODOVÁ, S.; HANSLIANOVA, M.; JUREN, T.; LIPOVÝ, B.; MAYER, J.; LENGEROVÁ, M.

Released

9. 2. 2022

Publisher

American Society for Microbiology

ISBN

2165-0497

Periodical

Microbiology spectrum

Year of study

10

Number

1

State

United States of America

Pages from

1

Pages to

10

Pages count

10

URL

https://journals.asm.org/doi/10.1128/spectrum.01817-21

Full text in the Digital Library

http://hdl.handle.net/11012/203954

BibTex

@article{BUT176720,
  author="Kristýna {Dufková} and Matěj {Bezdíček} and Kristina {Čuprová} and Dagmar {Pantůčková} and Markéta {Jakubíčková} and Eva {Brhelová} and Iva {Kocmanová} and Silvie {Hodová} and Markéta {Hanslianova} and Tomáš {Juren} and Břetislav {Lipový} and Jiří {Mayer} and Martina {Lengerová}",
  title="Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization",
  journal="Microbiology spectrum",
  year="2022",
  volume="10",
  number="1",
  pages="1--10",
  doi="10.1128/spectrum.01817-21",
  issn="2165-0497",
  url="https://journals.asm.org/doi/10.1128/spectrum.01817-21"
}