Publication detail

G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques

Vidláková P. Pivoňková H. Kejnovská I.Trnková L. Vorlíčková M. Fojta M. Havran L.

Original Title

G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques

Type

journal article in Web of Science

Language

English

Original Abstract

Electrochemical methods, particularly when applied in connection with mercury-containing electrodes, are excellent tools for studying nucleic acids structure and monitoring structural transitions. We studied the effect of the length of the central (dG)n stretch (varying from 0 to 15 guanine residues) in 15-mer oligodeoxynucleotides (ODN, G0 to G15) on their electrochemical and interfacial behavior at mercury and carbon electrodes. The intensity of guanine oxidation signal at the carbon electrode (peak Gox) was observed to increase continuously with number of guanines between 0 and 15, with only a slight positive shift for ODNs with seven or more guanines in the central segment. Very different effects were observed when the peak GHMDE was measured at the mercury electrode. Intensity of the latter signal increased with number of guanines up to G5, and decreased sharply with further elongation of the (dG)n stretch. CD spectroscopy and electrophoresis experiments revealed formation of parallel intermolecular quadruplex structures for ODNs containing five or more G residues. Further measurements made by cyclic and alternating-current voltammetry revealed a strong influence of the ODN structure on their behavior at electrically charged surfaces. © 2015 Springer-Verlag Berlin Heidelberg

Keywords

Electrochemical methods; G-quadruplex; Hanging mercury drop electrode; Oligonucleotides

Authors

Vidláková P.; Pivoňková H.; Kejnovská I.;Trnková L.; Vorlíčková M.; Fojta M.; Havran L.

Released

30. 5. 2021

ISBN

1618-2642

Periodical

Analytical and Bioanalytical Chemistry

Year of study

407

Number

12

State

Federal Republic of Germany

Pages from

5817

Pages to

5826

Pages count

10

URL

https://www.scopus.com/record/display.uri?eid=2-s2.0-84952826168&origin=inward&txGid=daf30a0832d4ac7af6b4e6142afa8380

BibTex

@article{BUT176965,
  author="Vidláková P. and Pivoňková H. and Kejnovská I. and Trnková L. and Vorlíčková M. and Fojta M. and Havran L.",
  title="G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques",
  journal="Analytical and Bioanalytical Chemistry",
  year="2021",
  volume="407",
  number="12",
  pages="5817--5826",
  doi="10.1007/s00216-015-8768-1",
  issn="1618-2642",
  url="https://www.scopus.com/record/display.uri?eid=2-s2.0-84952826168&origin=inward&txGid=daf30a0832d4ac7af6b4e6142afa8380"
}