3D-Printed COVID-19 immunosensors with electronic readout

journal article in Web of Science

English

3D printing technology has brought light in the fight against the COVID-19 global pandemic event through the decentralized and on-demand manufacture of different personal protective equipment and medical devices. Nonetheless, since this technology is still in an early stage, the use of 3D-printed electronic devices for antigen test developments is almost an unexplored field. Herein, a robust and general bottom-up biofunctionalization approach via surface engineering is reported aiming at providing the bases for the fabrication of the first 3D-printed COVID-19 immunosensor prototype with electronic readout. The 3D-printed COVID-19 immunosensor was constructed by covalently anchoring the COVID-19 recombinant protein on a 3D-printed graphene-based nanocomposite electrode surface. The electrical readout relies on impedimetrically monitoring changes at the electrode/electrolyte interface after interacting with the monoclonal COVID-19 antibody via competitive assay, fact that hinders the redox conversion of a benchmark redox marker. Overall, the developed 3D-printed system exhibits promising electroanalytical capabilities in both buffered and human serum samples, displaying an excellent linear response with a detection limit at trace levels (0.5 +/- 0.1 mu g.mL(-1)). Such achievements demonstrate advantage of light-of-speed distribution of 3D printing datafiles with localized point-of-care low-cost printing and bioelectronic devices to help contain the spread of emerging infectious diseases such as COVID-19. This technology is applicable to any post-COVID-19 SARS diseases.

3D-printed electrodes; SARS-CoV-2; Antigen test; Electrochemical detection; Surface engineering

MUÑOZ MARTIN, J.; PUMERA, M.

1. 12. 2021

ELSEVIER SCIENCE SA

LAUSANNE

1873-3212

CHEMICAL ENGINEERING JOURNAL

425

1

Swiss Confederation

131433-1

131433-2

8

https://www.sciencedirect.com/science/article/pii/S138589472103014X?via%3Dihub