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ŠAFAŘÍKOVÁ, E. EHLICH, J. STŘÍTESKÝ, S. VALA, M. WEITER, M. PACHERNÍK, J. KUBALA, L. VÍTEČEK, J.
Original Title
Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation
Type
journal article in Web of Science
Language
English
Original Abstract
Organic semiconductors are constantly gaining interest in regenerative medicine. Their tunable physico-chemical properties, including electrical conductivity, are very promising for the control of stem-cell differentiation. However, their use for combined material-based and electrical stimulation remains largely underexplored. Therefore, we carried out a study on whether a platform based on the conductive polymer poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) can be beneficial to the differentiation of mouse embryonic stem cells (mESCs). The platform was prepared using the layout of a standard 24-well cell-culture plate. Polyethylene naphthalate foil served as the substrate for the preparation of interdigitated gold electrodes by physical vapor deposition. The PEDOT:PSS pattern was fabricated by precise screen printing over the gold electrodes. The PEDOT:PSS platform was able to produce higher electrical current with the pulsed-direct-current (DC) electrostimulation mode (1 Hz, 200 mV/mm, 100 ms pulse duration) compared to plain gold electrodes. There was a dominant capacitive component. In proof-of-concept experiments, mESCs were able to respond to such electrostimulation by membrane depolarization and elevation of cytosolic calcium. Further, the PEDOT:PSS platform was able to upregulate cardiomyogenesis and potentially inhibit early neurogenesis per se with minor contribution of electrostimulation. Hence, the present work highlights the large potential of PEDOT:PSS in regenerative medicine.
Keywords
conductive polymer; PEDOT; PSS; screen print; embryonic stem cells; electrostimulation
Authors
ŠAFAŘÍKOVÁ, E.; EHLICH, J.; STŘÍTESKÝ, S.; VALA, M.; WEITER, M.; PACHERNÍK, J.; KUBALA, L.; VÍTEČEK, J.
Released
20. 1. 2022
Publisher
MDPI
Location
BASEL
ISBN
1422-0067
Periodical
International Journal of Molecular Sciences
Year of study
23
Number
3
State
Swiss Confederation
Pages from
1
Pages to
16
Pages count
URL
https://www.mdpi.com/1422-0067/23/3/1107
Full text in the Digital Library
http://hdl.handle.net/11012/204601
BibTex
@article{BUT177314, author="Eva {Šafaříková} and Jiří {Ehlich} and Stanislav {Stříteský} and Martin {Vala} and Martin {Weiter} and Jiří {Pacherník} and Lukáš {Kubala} and Jan {Víteček}", title="Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation", journal="International Journal of Molecular Sciences", year="2022", volume="23", number="3", pages="1--16", doi="10.3390/ijms23031107", issn="1422-0067", url="https://www.mdpi.com/1422-0067/23/3/1107" }