Publication detail

Insect Body Defence Reactions against Bee Venom: Do Adipokinetic Hormones Play a Role?

BODLÁKOVÁ, K. ČERNÝ, J. ŠTĚRBOVÁ, H. GURÁŇ, R. ZÍTKA, O. KODRÍK, D.

Original Title

Insect Body Defence Reactions against Bee Venom: Do Adipokinetic Hormones Play a Role?

Type

journal article in Web of Science

Language

English

Original Abstract

Bees originally developed their stinging apparatus and venom against members of their own species from other hives or against predatory insects. Nevertheless, the biological and biochemical response of arthropods to bee venom is not well studied. Thus, in this study, the physiological responses of a model insect species (American cockroach, Periplaneta americana) to honeybee venom were investigated. Bee venom toxins elicited severe stress (LD50 = 1.063 uL venom) resulting in a significant increase in adipokinetic hormones (AKHs) in the cockroach central nervous system and haemolymph. Venom treatment induced a large destruction of muscle cell ultrastructure, especially myofibrils and sarcomeres. Interestingly, co-application of venom with cockroach Peram-CAH-II AKH eliminated this effect. Envenomation modulated the levels of carbohydrates, lipids, and proteins in the haemolymph and the activity of digestive amylases, lipases, and proteases in the midgut. Bee venom significantly reduced vitellogenin levels in females. Dopamine and glutathione (GSH and GSSG) insignificantly increased after venom treatment. However, dopamine levels significantly increased after Peram-CAH-II application and after co-application with bee venom, while GSH and GSSG levels immediately increased after co-application. The results suggest a general reaction of the cockroach body to bee venom and at least a partial involvement of AKHs.

Keywords

adipokinetic hormone; American cockroach; dopamine; honey bee; melittin; metabolism; muscle ultrastructure; vitellogenin

Authors

BODLÁKOVÁ, K.; ČERNÝ, J.; ŠTĚRBOVÁ, H.; GURÁŇ, R.; ZÍTKA, O.; KODRÍK, D.

Released

31. 1. 2022

Publisher

MDPI

ISBN

2072-6651

Periodical

Toxins

Year of study

14

Number

1

State

Swiss Confederation

Pages from

1

Pages to

18

Pages count

18

URL

https://www.mdpi.com/2072-6651/14/1/11

Full text in the Digital Library

http://hdl.handle.net/11012/208106

BibTex

@article{BUT178332,
  author="Karolína {Bodláková} and Jan {Černý} and Helena {Štěrbová} and Roman {Guráň} and Ondřej {Zítka} and Dalibor {Kodrík}",
  title="Insect Body Defence Reactions against Bee Venom: Do Adipokinetic Hormones Play a Role?",
  journal="Toxins",
  year="2022",
  volume="14",
  number="1",
  pages="1--18",
  doi="10.3390/toxins14010011",
  issn="2072-6651",
  url="https://www.mdpi.com/2072-6651/14/1/11"
}