Publication detail

Calcined Hydroxyapatite with Collagen I Foam Promotes Human MSC Osteogenic Differentiation

BLAHNOVÁ, V. VOJTOVÁ, L. PAVLIŇÁKOVÁ, V. MUCHOVÁ, J. FILOVA, E.

Original Title

Calcined Hydroxyapatite with Collagen I Foam Promotes Human MSC Osteogenic Differentiation

Type

journal article in Web of Science

Language

English

Original Abstract

Collagen I-based foams were modified with calcined or noncalcined hydroxyapatite or calcium phosphates with various particle sizes and pores to monitor their effect on cell interactions. The resulting scaffolds thus differed in grain size, changing from nanoscale to microscopic, and possessed diverse morphological characteristics and resorbability. The materials' biological action was shown on human bone marrow MSCs. Scaffold morphology was identified by SEM. Using viability test, qPCR, and immunohistochemical staining, we evaluated the biological activity of all of the materials. This study revealed that the most suitable scaffold composition for osteogenesis induction is collagen I foam with calcined hydroxyapatite with a pore size of 360 +/- 130 mu m and mean particle size of 0.130 mu m. The expression of osteogenic markers RunX2 and ColI mRNA was promoted, and a strong synthesis of extracellular protein osteocalcin was observed. ColI/calcined HAP scaffold showed significant osteogenic potential, and can be easily manipulated and tailored to the defect size, which gives it great potential for bone tissue engineering applications.

Keywords

collagen; bioceramics; osteogenesis

Authors

BLAHNOVÁ, V.; VOJTOVÁ, L.; PAVLIŇÁKOVÁ, V.; MUCHOVÁ, J.; FILOVA, E.

Released

1. 4. 2022

Publisher

MDPI

Location

BASEL

ISBN

1422-0067

Periodical

International Journal of Molecular Sciences

Year of study

23

Number

8

State

Swiss Confederation

Pages from

1

Pages to

17

Pages count

17

URL

https://www.mdpi.com/1422-0067/23/8/4236

Full text in the Digital Library

http://hdl.handle.net/11012/208195

BibTex

@article{BUT178551,
  author="Veronika {Blahnová} and Lucy {Vojtová} and Veronika {Pavliňáková} and Johana {Muchová} and Eva {Filova}",
  title="Calcined Hydroxyapatite with Collagen I Foam Promotes Human MSC Osteogenic Differentiation",
  journal="International Journal of Molecular Sciences",
  year="2022",
  volume="23",
  number="8",
  pages="1--17",
  doi="10.3390/ijms23084236",
  issn="1422-0067",
  url="https://www.mdpi.com/1422-0067/23/8/4236"
}