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SMĚLÁ, D. CHANG, C.J. HROMÁDKO, L. MACÁK, J. BÍLKOVÁ, Z. TANIGUCHI, A.
Original Title
SiO2 Fibers of Two Lengths and Their Effect on Cellular Responses of Macrophage-like Cells
Type
journal article in Web of Science
Language
English
Original Abstract
The immunoreactivity or/and stress response can be induced by nanomaterials' different properties, such as size, shape, etc. These effects are, however, not yet fully understood. This study aimed to clarify the effects of SiO2 nanofibers (SiO(2)NFs) on the cellular responses of THP-1-derived macrophage-like cells. The effects of SiO(2)NFs with different lengths on reactive oxygen species (ROS) and pro-inflammatory cytokines TNF-alpha and IL-1 beta in THP-1 cells were evaluated. From the two tested lengths, it was only the L-SiO(2)NFs with a length approximate to 44 +/- 22 mu m that could induce ROS. Compared to this, only S-SiO(2)NFs with a length approximate to 14 +/- 17 mu m could enhance TNF-alpha and IL-1 beta expression. Our results suggested that L-SiO(2)NFs disassembled by THP-1 cells produced ROS and that the inflammatory reaction was induced by the uptake of S-SiO(2)NFs by THP-1 cells. The F-actin staining results indicated that SiO(2)NFs induced cell motility and phagocytosis. There was no difference in cytotoxicity between L- and S-SiO(2)NFs. However, our results suggested that the lengths of SiO(2)NFs induced different cellular responses.
Keywords
SiO2 nanofibers; cytotoxicity; immunoreactivity; THP-1-derived macrophage-like cells
Authors
SMĚLÁ, D.; CHANG, C.J.; HROMÁDKO, L.; MACÁK, J.; BÍLKOVÁ, Z.; TANIGUCHI, A.
Released
1. 7. 2022
Publisher
MDPI
Location
BASEL
ISBN
1420-3049
Periodical
MOLECULES
Year of study
27
Number
14
State
Swiss Confederation
Pages from
4456
Pages to
4468
Pages count
13
URL
https://www.mdpi.com/1420-3049/27/14/4456
BibTex
@article{BUT179258, author="SMĚLÁ, D. and CHANG, C.J. and HROMÁDKO, L. and MACÁK, J. and BÍLKOVÁ, Z. and TANIGUCHI, A.", title="SiO2 Fibers of Two Lengths and Their Effect on Cellular Responses of Macrophage-like Cells", journal="MOLECULES", year="2022", volume="27", number="14", pages="13", doi="10.3390/molecules27144456", issn="1420-3049", url="https://www.mdpi.com/1420-3049/27/14/4456" }