Publication detail

Cytokine expression by CD163+monocytes in healthy and Actinobacillus pleuropneumoniae-infected pigs

JAROŠOVÁ, R. ONDRÁČKOVÁ, P. LEVÁ, L. NEDBALCOVÁ, K. VÍCENOVÁ, M. MAŠEK, J. VOLF, J. GEBAUER, J. DO, T. GURÁŇ, R. SLÁDEK, Z. DOMINGUEZ, J. FALDYNA, M.

Original Title

Cytokine expression by CD163+monocytes in healthy and Actinobacillus pleuropneumoniae-infected pigs

Type

journal article in Web of Science

Language

English

Original Abstract

Distinct monocyte subpopulations have been previously described in healthy pigs and pigs experimentally infected with Actinobacillus pleuropneumoniae (APP). The CD163+ subpopulation of bone marrow (BM), peripheral blood (PB) and lung monocytes was found to play an important role in the inflammatory process. The inflammation is accompanied by elevation of inflammatory cytokines. The aim of the study was to evaluate the contribution of CD163+ monocytes and macrophages to cytokine production during APP-induced lung inflammation. Cytokine production was assessed by flow cytometry (FC) and quantitative PCR (qPCR) in CD163+ monocytes and by qPCR, immunohistochemistry/fluorescence in lungs and tracheobronchial lymph nodes (TBLN). Despite the systemic inflammatory response after APP infection, BM and PB CD163+ monocytes did not express elevated levels of a wide range of cytokines compared to control pigs. In contrast, significant amounts of IL-1 beta, IL-6, IL-8 and TNF-alpha were produced in lung lesions and IL-1 beta in the TBLN. At the protein level, TNF-alpha was expressed by both CD163+ monocytes and macrophages in lung lesions, whereas IL-1 beta, IL-6 and IL-8 expression was found only in CD163+ monocytes; no CD163+ macrophages were found to produce these cytokines. Furthermore, the quantification of CD163+ monocytes expressing the two cytokines IL-1 beta and IL-8 that were most elevated was performed. In lung lesions, 36.5% IL-1 beta positive CD163+ monocytes but only 18.3% IL-8 positive CD163+ monocytes were found. In conclusion, PB and BM CD163+ monocytes do not appear to contribute to the elevated cytokine levels in plasma. On the other hand, CD163+ monocytes contribute to inflammatory cytokine expression, especially IL-1 beta at the site of inflammation during the inflammatory process.

Keywords

Pig; Actinobacillus pleuropneumoniae; Monocyte; CD163; Cytokine

Authors

JAROŠOVÁ, R.; ONDRÁČKOVÁ, P.; LEVÁ, L.; NEDBALCOVÁ, K.; VÍCENOVÁ, M.; MAŠEK, J.; VOLF, J.; GEBAUER, J.; DO, T.; GURÁŇ, R.; SLÁDEK, Z.; DOMINGUEZ, J.; FALDYNA, M.

Released

20. 12. 2022

ISBN

0034-5288

Periodical

RESEARCH IN VETERINARY SCIENCE

Year of study

152

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

9

Pages count

9

URL

https://doi.org/10.1016/j.rvsc.2022.07.015

BibTex

@article{BUT179312,
  author="JAROŠOVÁ, R. and ONDRÁČKOVÁ, P. and LEVÁ, L. and NEDBALCOVÁ, K. and VÍCENOVÁ, M. and MAŠEK, J. and VOLF, J. and GEBAUER, J. and DO, T. and GURÁŇ, R. and SLÁDEK, Z. and DOMINGUEZ, J. and FALDYNA, M.",
  title="Cytokine expression by CD163+monocytes in healthy and Actinobacillus pleuropneumoniae-infected pigs",
  journal="RESEARCH IN VETERINARY SCIENCE",
  year="2022",
  volume="152",
  number="1",
  pages="1--9",
  doi="10.1016/j.rvsc.2022.07.015",
  issn="0034-5288",
  url="https://doi.org/10.1016/j.rvsc.2022.07.015"
}