Publication detail

Toward understanding the kinetics of disassembly of ferritins of varying origin and subunit composition

KRAUSOVÁ, K. CHAROUSOVÁ, M. KRATOCHVÍL, Z. TAKÁCSOVÁ, P. TESAŘOVÁ, B. SIVÁK, L. PEŠKOVÁ, M. SÚKUPOVÁ, M. ŽIVOTSKÁ, H. MAKOVICKÝ, P. YAMASHITA, I. OKAMOTO, N. HYNEK, D. HADDAD, Y. PEKAŘÍK, V. REX, S. HEGER, Z.

Original Title

Toward understanding the kinetics of disassembly of ferritins of varying origin and subunit composition

Type

journal article in Web of Science

Language

English

Original Abstract

Understanding the reversible reassembly properties of ferritins (FRTs) is crucial for enabling their applicability in nanomedicine. These properties include drug loading capabilities and also subsequent payload release in desired site-of-action. Thus, the presented study is focused on understanding the disassembly of recombinantly produced FRTs of varying origin and subunit composition, i.e. equine FRT composed of 22 L- and 2 H-subunits (EcaLH) or 24 L-subunits (EcaL), human FRT composed of 24 H-subunits (HsaH) and archaeal Pyrococcus furiosus FRT (Pfu). Disassembly was distinctly influenced by pH and ionic strength. Except for Pfu, the disassembly kinetics in acidic pH is rapid upon reaching an innate threshold, reaching the final state within minutes. Disassembly kinetics in basic pH is slower. Pfu is partially disassembled within the entire pH range. While equine FRT disassembles into free subunits, HsaH disassembles into subunit clusters. EcaL and Pfu form large aggregates during disassembly in mildly acidic pH, although basic pH causes partial disassembly without aggregation, suggesting usability for basic pH-triggered drug loading. We show that in human serum/plasma, FRTs readily form protein corona, hampering their uptake. Interestingly, we found out that archaeal Pfu likely exhibits similar receptor affinity as mammalian FRTs. Further, in vivo toxicity and biodistribution study of a single dose of FRTs demonstrated very low toxicity of FRTs and their preferential liver/kidney bioaccumulation.

Keywords

Archaeal ferritin; Continuous multimodal nanoparticle size; analysis; Mammalian ferritin; Native PAGE; Protein corona

Authors

KRAUSOVÁ, K.; CHAROUSOVÁ, M.; KRATOCHVÍL, Z.; TAKÁCSOVÁ, P.; TESAŘOVÁ, B.; SIVÁK, L.; PEŠKOVÁ, M.; SÚKUPOVÁ, M.; ŽIVOTSKÁ, H.; MAKOVICKÝ, P.; YAMASHITA, I.; OKAMOTO, N.; HYNEK, D.; HADDAD, Y.; PEKAŘÍK, V.; REX, S.; HEGER, Z.

Released

31. 8. 2022

ISBN

2352-9407

Periodical

Applied Materials Today

Year of study

28

Number

101535

State

Kingdom of the Netherlands

Pages from

1

Pages to

12

Pages count

12

URL

https://doi.org/10.1016/j.apmt.2022.101535

BibTex

@article{BUT179433,
  author="KRAUSOVÁ, K. and CHAROUSOVÁ, M. and KRATOCHVÍL, Z. and TAKÁCSOVÁ, P. and TESAŘOVÁ, B. and SIVÁK, L. and PEŠKOVÁ, M. and SÚKUPOVÁ, M. and ŽIVOTSKÁ, H. and MAKOVICKÝ, P. and YAMASHITA, I. and OKAMOTO, N. and HYNEK, D. and HADDAD, Y. and PEKAŘÍK, V. and REX, S. and HEGER, Z.",
  title="Toward understanding the kinetics of disassembly of ferritins of varying origin and subunit composition",
  journal="Applied Materials Today",
  year="2022",
  volume="28",
  number="101535",
  pages="1--12",
  doi="10.1016/j.apmt.2022.101535",
  issn="2352-9407",
  url="https://doi.org/10.1016/j.apmt.2022.101535"
}