Publication detail

Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells

BÁČOVÁ, J. KNOTEK, P. KOPECKÁ, K. HROMÁDKO, L. ČAPEK, J. NÝVLTOVÁ, P. BRŮČKOVÁ, L. SCHRÖTEROVÁ, L. ŠESTÁKOVÁ, B. PALARČÍK, J. MOTOLA, M. ČÍŽKOVÁ, D. BEZROUK, A. HANDL, J. FIALA, Z. RUDOLF, E. BÍLKOVÁ, Z. MACÁK, J. ROUŠAR, T.

Original Title

Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells

Type

journal article in Web of Science

Language

English

Original Abstract

Purpose: Titanium dioxide nanoparticles, 25 nm in size of crystallites (TiO2 P25), are among the most produced nanomaterials worldwide. The broad use of TiO2 P25 in material science has implied a request to evaluate their biological effects, especially in the lungs. Hence, the pulmonary A549 cell line has been used to estimate the effects of TiO2 P25. However, the reports have provided dissimilar results on caused toxicity. Surprisingly, the physicochemical factors influencing TiO2 P25 action in biological models have not been evaluated in most reports. Thus, the objective of the present study is to characterize the preparation of TiO2 P25 for biological testing in A549 cells and to evaluate their biological effects.Methods: We determined the size and crystallinity of TiO2 P25. We used four techniques for TiO2 P25 dispersion. We estimated the colloid stability of TiO2 P25 in distilled water, isotonic NaCl solution, and cell culture medium. We applied the optimal dispersion conditions for testing the biological effects of TiO2 P25 (0-100 mu g.mL-1) in A549 cells using biochemical assays (dehydrogenase activity, glutathione levels) and microscopy.Results: We found that the use of fetal bovine serum in culture medium is essential to maintain sufficient colloid stability of dispersed TiO2 P25. Under these conditions, TiO2 P25 were unable to induce a significant impairment of A549 cells according to the results of biochemical and microscopy evaluations. When the defined parameters for the use of TiO2 P25 in A549 cells were met, similar results on the biological effects of TiO2 P25 were obtained in two independent cell laboratories.Conclusion: We optimized the experimental conditions of TiO2 P25 preparation for toxicity testing in A549 cells. The results presented here on TiO2 P25-induced cellular effects are reproducible. Therefore, our results can be helpful for other researchers using TiO2 P25 as a reference material.

Keywords

titanium dioxide; nanoparticles; P25; nanotoxicity; A549 cells; dispersion

Authors

BÁČOVÁ, J.; KNOTEK, P.; KOPECKÁ, K.; HROMÁDKO, L.; ČAPEK, J.; NÝVLTOVÁ, P.; BRŮČKOVÁ, L.; SCHRÖTEROVÁ, L.; ŠESTÁKOVÁ, B.; PALARČÍK, J.; MOTOLA, M.; ČÍŽKOVÁ, D.; BEZROUK, A.; HANDL, J.; FIALA, Z.; RUDOLF, E.; BÍLKOVÁ, Z.; MACÁK, J.; ROUŠAR, T.

Released

30. 9. 2022

Publisher

Dove Press

Location

ALBANY

ISBN

1178-2013

Periodical

International Journal of Nanomedicine

Year of study

17

Number

1

State

New Zealand

Pages from

4211

Pages to

4225

Pages count

15

URL

https://www.dovepress.com/evaluating-the-use-of-tio2-nanoparticles-for-toxicity-testing-in-pulmo-peer-reviewed-fulltext-article-IJN

Full text in the Digital Library

http://hdl.handle.net/11012/208681

BibTex

@article{BUT179712,
  author="Jana {Báčová} and Petr {Knotek} and Kateřina {Kopecká} and Luděk {Hromádko} and Jan {Čapek} and Pavlína {Nývltová} and Lenka {Brůčková} and Ladislava {Schröterová} and Blanka {Šestáková} and Jiří {Palarčík} and Martin {Motola} and Dana {Čížková} and Aleš {Bezrouk} and Jiří {Handl} and Zdeněk {Fiala} and Emil {Rudolf} and Zuzana {Bílková} and Jan {Macák} and Tomáš {Roušar}",
  title="Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells",
  journal="International Journal of Nanomedicine",
  year="2022",
  volume="17",
  number="1",
  pages="4211--4225",
  doi="10.2147/IJN.S374955",
  issn="1178-2013",
  url="https://www.dovepress.com/evaluating-the-use-of-tio2-nanoparticles-for-toxicity-testing-in-pulmo-peer-reviewed-fulltext-article-IJN"
}