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BÁČOVÁ, J. KNOTEK, P. KOPECKÁ, K. HROMÁDKO, L. ČAPEK, J. NÝVLTOVÁ, P. BRŮČKOVÁ, L. SCHRÖTEROVÁ, L. ŠESTÁKOVÁ, B. PALARČÍK, J. MOTOLA, M. ČÍŽKOVÁ, D. BEZROUK, A. HANDL, J. FIALA, Z. RUDOLF, E. BÍLKOVÁ, Z. MACÁK, J. ROUŠAR, T.
Original Title
Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells
Type
journal article in Web of Science
Language
English
Original Abstract
Purpose: Titanium dioxide nanoparticles, 25 nm in size of crystallites (TiO2 P25), are among the most produced nanomaterials worldwide. The broad use of TiO2 P25 in material science has implied a request to evaluate their biological effects, especially in the lungs. Hence, the pulmonary A549 cell line has been used to estimate the effects of TiO2 P25. However, the reports have provided dissimilar results on caused toxicity. Surprisingly, the physicochemical factors influencing TiO2 P25 action in biological models have not been evaluated in most reports. Thus, the objective of the present study is to characterize the preparation of TiO2 P25 for biological testing in A549 cells and to evaluate their biological effects.Methods: We determined the size and crystallinity of TiO2 P25. We used four techniques for TiO2 P25 dispersion. We estimated the colloid stability of TiO2 P25 in distilled water, isotonic NaCl solution, and cell culture medium. We applied the optimal dispersion conditions for testing the biological effects of TiO2 P25 (0-100 mu g.mL-1) in A549 cells using biochemical assays (dehydrogenase activity, glutathione levels) and microscopy.Results: We found that the use of fetal bovine serum in culture medium is essential to maintain sufficient colloid stability of dispersed TiO2 P25. Under these conditions, TiO2 P25 were unable to induce a significant impairment of A549 cells according to the results of biochemical and microscopy evaluations. When the defined parameters for the use of TiO2 P25 in A549 cells were met, similar results on the biological effects of TiO2 P25 were obtained in two independent cell laboratories.Conclusion: We optimized the experimental conditions of TiO2 P25 preparation for toxicity testing in A549 cells. The results presented here on TiO2 P25-induced cellular effects are reproducible. Therefore, our results can be helpful for other researchers using TiO2 P25 as a reference material.
Keywords
titanium dioxide; nanoparticles; P25; nanotoxicity; A549 cells; dispersion
Authors
BÁČOVÁ, J.; KNOTEK, P.; KOPECKÁ, K.; HROMÁDKO, L.; ČAPEK, J.; NÝVLTOVÁ, P.; BRŮČKOVÁ, L.; SCHRÖTEROVÁ, L.; ŠESTÁKOVÁ, B.; PALARČÍK, J.; MOTOLA, M.; ČÍŽKOVÁ, D.; BEZROUK, A.; HANDL, J.; FIALA, Z.; RUDOLF, E.; BÍLKOVÁ, Z.; MACÁK, J.; ROUŠAR, T.
Released
30. 9. 2022
Publisher
Dove Press
Location
ALBANY
ISBN
1178-2013
Periodical
International Journal of Nanomedicine
Year of study
17
Number
1
State
New Zealand
Pages from
4211
Pages to
4225
Pages count
15
URL
https://www.dovepress.com/evaluating-the-use-of-tio2-nanoparticles-for-toxicity-testing-in-pulmo-peer-reviewed-fulltext-article-IJN
Full text in the Digital Library
http://hdl.handle.net/11012/208681
BibTex
@article{BUT179712, author="Jana {Báčová} and Petr {Knotek} and Kateřina {Kopecká} and Luděk {Hromádko} and Jan {Čapek} and Pavlína {Nývltová} and Lenka {Brůčková} and Ladislava {Schröterová} and Blanka {Šestáková} and Jiří {Palarčík} and Martin {Motola} and Dana {Čížková} and Aleš {Bezrouk} and Jiří {Handl} and Zdeněk {Fiala} and Emil {Rudolf} and Zuzana {Bílková} and Jan {Macák} and Tomáš {Roušar}", title="Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells", journal="International Journal of Nanomedicine", year="2022", volume="17", number="1", pages="4211--4225", doi="10.2147/IJN.S374955", issn="1178-2013", url="https://www.dovepress.com/evaluating-the-use-of-tio2-nanoparticles-for-toxicity-testing-in-pulmo-peer-reviewed-fulltext-article-IJN" }