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VYTISKOVÁ, K. OBOŘILOVÁ, R. NOVOTNÝ, K. FARKA, Z. SKLÁDAL, P. KAISER, J. POŘÍZKA, P.
Original Title
Detection of biomolecules labeled with photon-upconversion nanoparticles by laser-induced breakdown spectroscopy
Type
abstract
Language
English
Original Abstract
In this work we investigate the laser ablation with the use of different laser pulse wavelengths (266, 532, and 1064 nm) and fluences. Moreover, single pulse (SP) and double pulse (DP) LIBS arrangements were compared with respect to the enhancement of the readout sensitivity. We have deployed a simplified sample preprocessing when focusing solely on the analysis of UCNPs dopped with ytrium deposited on microscope slide. Obtained results suggest a great potential to meet immunohistochemistry (IHC) and immunocytochemistry (ICC) readouts needs.
Keywords
Tag-LIBS;Photon-upconversion nanopartilces;Laser-induced Breakdown Spectroscopy;Immunocytochemistry
Authors
VYTISKOVÁ, K.; OBOŘILOVÁ, R.; NOVOTNÝ, K.; FARKA, Z.; SKLÁDAL, P.; KAISER, J.; POŘÍZKA, P.
Released
1. 9. 2022
Publisher
Spektroskopická společnost Jana Marka Marci
Location
Brno
ISBN
978-80-88195-41-2
Book
ESAS-CSSC 2022 BOOK OF ABSTRACTS
Pages from
154
Pages to
Pages count
246
BibTex
@misc{BUT180076, author="Karolína {Vytisková} and Radka {Obořilová} and Karel {Novotný} and Zdeněk {Farka} and Petr {Skládal} and Jozef {Kaiser} and Pavel {Pořízka}", title="Detection of biomolecules labeled with photon-upconversion nanoparticles by laser-induced breakdown spectroscopy", booktitle="ESAS-CSSC 2022 BOOK OF ABSTRACTS", year="2022", pages="154--154", publisher="Spektroskopická společnost Jana Marka Marci", address="Brno", isbn="978-80-88195-41-2", note="abstract" }