Publication detail

Laser-Induced Breakdown Spectroscopy as a Readout Method for Detection of Biomolecules Labeled with Photon-Upconversion Nanoparticles

VYTISKOVÁ, K. OBOŘILOVÁ, R. NOVOTNÝ, K. FARKA, Z. POŘÍZKA, P. KAISER, J.

Original Title

Laser-Induced Breakdown Spectroscopy as a Readout Method for Detection of Biomolecules Labeled with Photon-Upconversion Nanoparticles

Type

abstract

Language

English

Original Abstract

In this work the laser ablation with the use of different laser pulse wavelengths (266, 532 and 1064 nm) and fluences were investigated. Furthermore, the single pulse (SP) and double pulse (DP) LIBS arrangements were compared. To focus wholly on analysis simplified sample processing was deployed and therefore UCNPs dopped with erbium and ytterbium deposited on microscope slide were used as samples. The best lateral resolution trade-off and the best yttrium signal-to-noise ratio were obtained using the combination of collinear DP LIBS setting with two laser pulses operating at 1064 nm wavelengths. Obtained results confirmed that LIBS suggests a big potential to meet IHC and ICC readouts needs.

Keywords

Tag-LIBS;Immunocytochemistry;Nanoparticles;Photon-Upconversion nanoparticles

Authors

VYTISKOVÁ, K.; OBOŘILOVÁ, R.; NOVOTNÝ, K.; FARKA, Z.; POŘÍZKA, P.; KAISER, J.

Released

3. 10. 2022

Pages from

281

Pages to

281

Pages count

450

URL

https://scixconference.org/resources/Documents/SciX%202022%20Abstract%20Book.pdf

BibTex

@misc{BUT180079,
  author="Karolína {Vytisková} and Radka {Obořilová} and Karel {Novotný} and Zdeněk {Farka} and Pavel {Pořízka} and Jozef {Kaiser}",
  title="Laser-Induced Breakdown Spectroscopy as a Readout Method for Detection
of Biomolecules Labeled with Photon-Upconversion Nanoparticles",
  booktitle="SciX 2022 Abstract book",
  year="2022",
  pages="281--281",
  url="https://scixconference.org/resources/Documents/SciX%202022%20Abstract%20Book.pdf",
  note="abstract"
}