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GOMEZ PEREZ, I. SULLEIRO, M. DOLEČKOVÁ, A. PIZÚROVÁ, N. MEDALOVÁ, J. BEDNAŘÍK, A. PREISLER, J. NEČAS, D. ZAJÍČKOVÁ, L.
Original Title
Structure elucidation of multicolor emissive graphene quantum dots towards cell guidance
Type
journal article in Web of Science
Language
English
Original Abstract
Graphene quantum dots (GQDs) can become excellent bioimaging tools when tuned to emit at larger wavelengths due to the minimal tissue absorbance and emission in this range. Tuning the GQD structure can help but understanding the chemical structure responsible for their properties remains challenging. Herein, we elucidated the structure of GQDs synthesized from glucose and ammonium hydroxide using a fast microwave-assisted hydrothermal protocol. Remarkably, these GQDs exhibited emission from the NUV-Vis up to the NIR range. The structure and chemical composition were elucidated using advanced NMR techniques, such as two-dimensional nuclear magnetic resonance, combined with traditional spectroscopy and electron microscopy. The graphitic core composed of pyrazines presented localized defects and lower rotation mobility compared with their edges that were mainly formed by hydroxyl, acid, and amine functional groups, which paved the way for the observed multicolor red-shifted fluorescence emission. Confocal laser scanning microscopy revealed functional cell imaging in a wide spectral range of fluorescence from bright purple to red, confirming the uptake of GQDs by the cells without any observable toxicity. The non-cytotoxicity was further proved by the chemiluminescence cell viability adenosine triphosphate (ATP) assay. Combined with the tunable GQD emission, it gives them the potential to act as bioimaging carriers starting a new phase for their use in vivo.
Keywords
FACILE SYNTHESIS; CARBON DOTS; 2ND WINDOW; PHOTOLUMINESCENCE; NANODOTS; OXIDE; NITROGEN; YIELD; MOLECULES; REDUCTION
Authors
GOMEZ PEREZ, I.; SULLEIRO, M.; DOLEČKOVÁ, A.; PIZÚROVÁ, N.; MEDALOVÁ, J.; BEDNAŘÍK, A.; PREISLER, J.; NEČAS, D.; ZAJÍČKOVÁ, L.
Released
17. 1. 2022
Publisher
ROYAL SOC CHEMISTRY
Location
CAMBRIDGE
ISBN
2052-1537
Periodical
MATERIALS CHEMISTRY FRONTIERS
Year of study
6
Number
2
State
United Kingdom of Great Britain and Northern Ireland
Pages from
145
Pages to
154
Pages count
10
URL
https://pubs.rsc.org/en/content/articlelanding/2022/QM/D1QM01126J
BibTex
@article{BUT182337, author="Inmaculada Jennifer {Gomez Perez} and Manuel Vázquez {Sulleiro} and Anna {Dolečková} and Naděžda {Pizúrová} and Jiřina {Medalová} and Antonín {Bednařík} and Jan {Preisler} and David {Nečas} and Lenka {Zajíčková}", title="Structure elucidation of multicolor emissive graphene quantum dots towards cell guidance", journal="MATERIALS CHEMISTRY FRONTIERS", year="2022", volume="6", number="2", pages="145--154", doi="10.1039/d1qm01126j", issn="2052-1537", url="https://pubs.rsc.org/en/content/articlelanding/2022/QM/D1QM01126J" }