Publication detail

Infrared Laser Desorption of Intact Nanoparticles for Digital Tissue Imaging

STIBOREK, M. JINDŘICHOVÁ, L. MELIORISOVÁ, S. BEDNAŘÍK, A. PRYSIAZHNYI, V. KROUPA, J. HOUŠKA, P. ADAMOVÁ, B. NAVRÁTILOVÁ, J. KANICKÝ, V. PREISLER, J.

Original Title

Infrared Laser Desorption of Intact Nanoparticles for Digital Tissue Imaging

Type

journal article in Web of Science

Language

English

Original Abstract

We report a new technique for the digital mapping of biomarkers in tissues based on desorption and counting intact gold nanoparticle (Au NP) tags using infrared laser ablation single-particle inductively coupled plasma mass spectrometry (IR LA SP ICP MS). In contrast to conventional UV laser ablation, Au NPs are not disintegrated during the desorption process due to their low absorption at 2940 nm. A mass spectrometer detects up to 83% of Au NPs. The technique is demonstrated on mapping a proliferation marker, nuclear protein Ki-67, in three-dimensional (3D) aggregates of colorectal carcinoma cells, and the results are compared with confocal fluorescence microscopy and UV LA ICP MS. Precise counting of 20 nm Au NPs with a single-particle detection limit in each pixel by the new approach generates sharp distribution maps of a specific biomarker in the tissue. Advantageously, the desorption of Au NPs from regions outside the tissue is strongly suppressed. The developed methodology promises multiplex mapping of low-abundant biomarkers in numerous biological and medical applications using multielemental mass spectrometers.

Keywords

Desorption; Fluorescence microscopy; Gold nanoparticles; Inductively coupled plasma mass spectrometry; Infrared lasers; Laser ablation; Mapping; Mass spectrometers; Medical applications; Tissue

Authors

STIBOREK, M.; JINDŘICHOVÁ, L.; MELIORISOVÁ, S.; BEDNAŘÍK, A.; PRYSIAZHNYI, V.; KROUPA, J.; HOUŠKA, P.; ADAMOVÁ, B.; NAVRÁTILOVÁ, J.; KANICKÝ, V.; PREISLER, J.

Released

27. 12. 2022

Publisher

AMER CHEMICAL SOC

Location

WASHINGTON

ISBN

0003-2700

Periodical

ANALYTICAL CHEMISTRY

Year of study

94

Number

51

State

United States of America

Pages from

18114

Pages to

18120

Pages count

7

URL

https://pubs-acs-org.ezproxy.lib.vutbr.cz/doi/full/10.1021/acs.analchem.2c05216

BibTex

@article{BUT182555,
  author="Marek {Stiborek} and Lenka {Jindřichová} and Stanislava {Meliorisová} and Antonín {Bednařík} and Vadym {Prysiazhnyi} and Jiří {Kroupa} and Pavel {Houška} and Barbora {Adamová} and Jarmila {Navrátilová} and Viktor {Kanický} and Jan {Preisler}",
  title="Infrared Laser Desorption of Intact Nanoparticles for Digital Tissue Imaging",
  journal="ANALYTICAL CHEMISTRY",
  year="2022",
  volume="94",
  number="51",
  pages="18114--18120",
  doi="10.1021/acs.analchem.2c05216",
  issn="0003-2700",
  url="https://pubs-acs-org.ezproxy.lib.vutbr.cz/doi/full/10.1021/acs.analchem.2c05216"
}