Publication detail

Kpkt killer toxin: a promising option for the manage-ment of microbial contaminations in the wine and food industry

MÁROVÁ, I.

Original Title

Kpkt killer toxin: a promising option for the manage-ment of microbial contaminations in the wine and food industry

Type

abstract

Language

English

Original Abstract

Yeast killer toxins are naturally secreted glycoproteins that kill microorganisms of agrifood interest and microbial pat-hogens through a toxin-receptor-mediated mechanism. Among yeast killer toxins, the one produced by Tetrapi-sispora phaffii, named Kpkt, is a 33 kDa β-glucanase that, being active on Kloeckera/Hanseniaspora, Saccharomyco-des and Zygosaccharomyces yeast, may find application in the management of microbial contamination in grape must and sweet beverages. However, as for other killer toxins, the industrial exploitation of Kpkt has been often proposed, but never realized. This is possibly due, among the others, to the lack of studies regarding their effect on the consumers, and to their limited production by the native producers. Considering that the utilization of recombinant enzymes is admitted by the food industry, KpKt coding gene was expressed in Pichia pastoris and the spectrum of action of the recombinant toxin was thoroughly evaluated on microbial targets and non-target eukaryotic models. Heterologous expression resulted in a 50-fold increase in the production of Kpkt. This was active on a rich portfolio of food-related yeast and bacteria and proved harmless on human keratinocytes HaCaT cells thus appearing a promising option for the management of microbial contamination in the wine and food industry

Keywords

killer toxin, natural antimicrobials, β-glucanase

Authors

MÁROVÁ, I.

Released

1. 2. 2023

Publisher

Sciendo

Location

Italy

ISBN

2564-615X

Book

The Eurobiotech Journal

Edition

1

Edition number

7

ISBN

2564-615X

Periodical

The EuroBiotech Journal

Year of study

7

Number

1

State

Republic of Italy

Pages from

27

Pages to

27

Pages count

1

URL

https://sciendo.com/article/10.2478/ebtj-2023-0003

BibTex

@misc{BUT182972,
  author="Ivana {Márová}",
  title="Kpkt  killer  toxin:  a  promising  option  for  the  manage-ment  of  microbial  contaminations  in  the  wine  and  food  industry",
  booktitle="The Eurobiotech Journal",
  year="2023",
  series="1",
  edition="7",
  journal="The EuroBiotech Journal",
  volume="7",
  number="1",
  pages="27--27",
  publisher="Sciendo",
  address="Italy",
  doi="DOI: 10.2478/ebtj-2023-0003",
  isbn="2564-615X",
  issn="2564-615X",
  url="https://sciendo.com/article/10.2478/ebtj-2023-0003",
  note="abstract"
}