Publication detail

Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells

PARTOVI NASR, M.

Original Title

Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells

Type

journal article - other

Language

English

Original Abstract

Recently, the translational application of noncoding RNAs is accelerated dramatically. In this regard, discovering therapeutic roles of microRNAs by developing synthetic RNA and vector-based RNA is attracting attention. Here, we studied the effect of BMP2 and miR-424 on the osteogenesis of Wharton’s jelly-derived stem cells (WJSCs). For this purpose, human BMP2 and miR-424 DNA codes were cloned in the third generation of lentiviral vectors and then used for HEK-293T cell transfection. Lentiviral plasmids contained miR424, BMP-2, miR424-BMP2, green fluorescent protein (GFP) genes, and helper vectors. The recombinant lentiviral particles transduced the WJSCs, and the osteogenesis was evaluated by real-time PCR, Western blot, Alizarin Red staining, and alkaline phosphatase enzyme activity. According to the results, there was a significant increase in the expression of the BMP2 gene and secretion of Osteocalcin protein in the group of miR424-BMP2. Moreover, the amount of dye deposition in Alizarin Red staining and alkaline phosphatase activity was significantly higher in the mentioned group (p<0.05). Thus, the current study results clarify the efficacy of gene therapy by miR424-BMP2 vectors for bone tissue engineering. These data could help guide the development of gene therapy-based protocols for bone tissue engineering.

Keywords

BMP2,WJSCs,miR-424

Authors

PARTOVI NASR, M.

Released

11. 8. 2021

Publisher

BioMed Research International

ISBN

2314-6141

Periodical

BioMed Research International

Year of study

2021

Number

7031492

State

United States of America

Pages from

11

Pages to

21

Pages count

10

URL

https://www.hindawi.com/journals/bmri/2021/7031492/

BibTex

@article{BUT183043,
  author="Minoo {Partovi Nasr} and Asghar {Fallah} and Zahra {Jamali} and Marziyeh {Aghazadeh} and Leila {Roshan gar} and Akbar {Farjad far}",
  title="Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells",
  journal="BioMed Research International",
  year="2021",
  volume="2021",
  number="7031492",
  pages="11--21",
  doi="10.1155/2021/7031492",
  issn="2314-6141",
  url="https://www.hindawi.com/journals/bmri/2021/7031492/"
}