Publication detail

Corynebacterium antarcticum sp. nov., Corynebacterium marambiense sp. nov., Corynebacterium meridianum sp. nov., and Corynebacterium pygosceleis sp. nov., isolated from Adelie penguins (Pygoscelis adeliae)

ŠVEC, P. BUSSE, H. SEDLÁŘ, K. MUSILOVÁ, J. KRÁLOVÁ, S. STAŇKOVÁ, E. ŠEDO, O. KRSEK, D. KOUBLOVÁ, V. KRIŠTOFOVÁ, L. SEDLÁČEK, I.

Original Title

Corynebacterium antarcticum sp. nov., Corynebacterium marambiense sp. nov., Corynebacterium meridianum sp. nov., and Corynebacterium pygosceleis sp. nov., isolated from Adelie penguins (Pygoscelis adeliae)

Type

journal article in Web of Science

Language

English

Original Abstract

A taxonomic study was conducted on 16 bacterial strains isolated from wild Adelie penguins (Pygoscelis adeliae) from Seymour (Marambio) Island and James Ross Island. An initial screening by repetitive sequence-based PCR fingerprinting divided the strains studied into four coherent groups. Phylogenetic analysis based on 16S rRNA gene sequences assigned all groups to the genus Corynebacterium and showed that Corynebacterium glyciniphilum and Corynebacterium terpenotabidum were the closest species with 16S rRNA gene sequence similarities between 95.4 % and 96.5 %. Further examination of the strains studied with ribotyping, MALDI-TOF mass spectrometry, comprehensive biotyping and calculation of average nucleotide identity and digital DNA-DNA hybridisation values confirmed the separation of the four groups from each other and from the other Corynebacterium species. Chemotaxonomically, the four strains P5828T, P5850T, P6136T, P7210T representing the studied groups were characterised by C16:0 and C18:1 omega 9c as the major fatty acids, by the presence of meso-diaminopimelic acid in the peptidoglycan, the presence of corynemycolic acids and a quinone system with the predominant menaquinone MK-9(H2). The results of this study show that the strains studied represent four new species of the genus Corynebacterium, for which the names Corynebacterium antarcticum sp. nov. (type strain P5850T = CCM 8835T = LMG 30620T), Corynebacterium marambiense sp. nov. (type strain P5828T = CCM 8864T = LMG 31626T), Corynebacterium meridianum sp. nov. (type strain P6136T = CCM 8863T = LMG 31628T) and Corynebacterium pygosceleis sp. nov. (type strain P7210T = CCM 8836T = LMG 30621T) are proposed.

Keywords

Corynebacterium; Taxonomy; sp; nov; Adelie penguin; Antarctica

Authors

ŠVEC, P.; BUSSE, H.; SEDLÁŘ, K.; MUSILOVÁ, J.; KRÁLOVÁ, S.; STAŇKOVÁ, E.; ŠEDO, O.; KRSEK, D.; KOUBLOVÁ, V.; KRIŠTOFOVÁ, L.; SEDLÁČEK, I.

Released

16. 1. 2023

Publisher

ELSEVIER GMBH

Location

MUNICH

ISBN

0723-2020

Periodical

SYSTEMATIC AND APPLIED MICROBIOLOGY

Year of study

46

Number

1

State

Federal Republic of Germany

Pages count

14

URL

https://www.sciencedirect.com/science/article/pii/S0723202022000972

BibTex

@article{BUT183382,
  author="Pavel {Švec} and Hans-Jürgen {Busse} and Karel {Sedlář} and Jana {Musilová} and Stanislava {Králová} and Eva {Staňková} and Ondrej {Šedo} and Daniel {Krsek} and Vendula {Koublová} and Lucie {Krištofová} and Ivo {Sedláček}",
  title="Corynebacterium antarcticum sp. nov., Corynebacterium marambiense sp. nov., Corynebacterium meridianum sp. nov., and Corynebacterium pygosceleis sp. nov., isolated from Adelie penguins (Pygoscelis adeliae)",
  journal="SYSTEMATIC AND APPLIED MICROBIOLOGY",
  year="2023",
  volume="46",
  number="1",
  pages="14",
  doi="10.1016/j.syapm.2022.126390",
  issn="0723-2020",
  url="https://www.sciencedirect.com/science/article/pii/S0723202022000972"
}