Publication detail

Metabolome plasticity in 241 Arabidopsis thaliana accessions reveals evolutionary cold adaptation processes

WEISZMANN, J. WALTHER, D. CLAUW, P. BACK, G. GUNIS, J. REICHARDT, I. KOEMEDA, S. JEZ, J. NORDBORG, M. PIERDIES, I. SCHWARZEROVÁ, J. NÄGELE, T. WECKWERTH, W.

Original Title

Metabolome plasticity in 241 Arabidopsis thaliana accessions reveals evolutionary cold adaptation processes

Type

journal article in Web of Science

Language

English

Original Abstract

Acclimation and adaptation of metabolism to a changing environment are key processes for plant survival and reproductive success. In the present study, 241 natural accessions of Arabidopsis (Arabidopsis thaliana) were grown under two different temperature regimes, 16 °C and 6 °C, and growth parameters were recorded, together with metabolite profiles, to investigate the natural genome × environment effects on metabolome variation. The plasticity of metabolism, which was captured by metabolic distance measures, varied considerably between accessions. Both relative growth rates and metabolic distances were predictable by the underlying natural genetic variation of accessions. Applying machine learning methods, climatic variables of the original growth habitats were tested for their predictive power of natural metabolic variation among accessions. We found specifically habitat temperature during the first quarter of the year to be the best predictor of the plasticity of primary metabolism, indicating habitat temperature as the causal driver of evolutionary cold adaptation processes. Analyses of epigenome- and genome-wide associations revealed accession-specific differential DNA-methylation levels as potentially linked to the metabolome and identified FUMARASE2 as strongly associated with cold adaptation in Arabidopsis accessions. These findings were supported by calculations of the biochemical Jacobian matrix based on variance and covariance of metabolomics data, which revealed that growth under low temperatures most substantially affects the accession-specific plasticity of fumarate and sugar metabolism. Our findings indicate that the plasticity of metabolic regulation is predictable from the genome and epigenome and driven evolutionarily by Arabidopsis growth habitats.

Keywords

Arabidopsis thaliana, Metabolomics, Evolution, Adaption, GWAS

Authors

WEISZMANN, J.; WALTHER, D.; CLAUW, P.; BACK, G.; GUNIS, J.; REICHARDT, I.; KOEMEDA, S.; JEZ, J.; NORDBORG, M.; PIERDIES, I.; SCHWARZEROVÁ, J.; NÄGELE, T.; WECKWERTH, W.

Released

23. 5. 2023

Publisher

Oxford University Press

ISBN

0032-0889

Periodical

PLANT PHYSIOLOGY

Year of study

192

Number

2

State

United States of America

Pages from

980

Pages to

1000

Pages count

21

URL

https://academic.oup.com/plphys/article/193/2/980/7176103

Full text in the Digital Library

http://hdl.handle.net/11012/244948

BibTex

@article{BUT183866,
  author="Jakob {Weiszmann} and Dirk {Walther} and Pieter {Clauw} and Georg {Back} and Joanna {Gunis} and Ilka {Reichardt} and Stefanie {Koemeda} and Jakub {Jez} and Magnus {Nordborg} and Jana {Schwarzerová} and Iro {Pierdies} and Thomas {Nägele} and Wolfram {Weckwerth}",
  title="Metabolome plasticity in 241 Arabidopsis thaliana accessions reveals evolutionary cold adaptation processes",
  journal="PLANT PHYSIOLOGY",
  year="2023",
  volume="192",
  number="2",
  pages="980--1000",
  doi="10.1093/plphys/kiad298",
  issn="0032-0889",
  url="https://academic.oup.com/plphys/article/193/2/980/7176103"
}