Publication detail

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica

NOVÁKOVÁ, D. KOUBLOVÁ, V. SEDLÁŘ, K. STAŇKOVÁ, E. KRÁLOVÁ, S. ŠVEC, P. NEUMANN-SCHAAL, M. WOLF, J. KOUDELKOVÁ, S. BARTÁK, M. SEDLÁČEK, I.

Original Title

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica

Type

journal article in Web of Science

Language

English

Original Abstract

A polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence analysis using the rpoB, rpoD, and gyrB genes. Genome sequence comparison of P2653T with the most related P. graminis type strain DSM 11363T revealed an average nucleotide identity of 92.1% and a digital DNA-DNA hybridization value of 46.6%. The major fatty acids for all Antarctic strains were C16:0, Summed Feature 3 (C16:1 omega 7c/C16:1 omega 6c) and Summed Feature 8 (C18:1 omega 7c/C18:1 omega 6c). The predominant respiratory quinone was Q-9, and the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol. The regolith strains could be differentiated from related species by the absence of arginine dihydrolase, ornithine and lysine decarboxylase and by negative tyrosine hydrolysis. The results of this polyphasic study allowed the genotypic and phenotypic differentiation of four analysed strains from the closest related species, which confirmed that the strains represent a novel species within the genus Pseudomonas, for which the name Pseudomonas petrae sp. nov. is proposed with P2653T (CCM 8850T = DSM 112068T = LMG 30619T) as the type strain.

Keywords

Pseudomonas; P. petrae sp. nov; Antarctica; Regolith; Species description; Taxonomy

Authors

NOVÁKOVÁ, D.; KOUBLOVÁ, V.; SEDLÁŘ, K.; STAŇKOVÁ, E.; KRÁLOVÁ, S.; ŠVEC, P.; NEUMANN-SCHAAL, M.; WOLF, J.; KOUDELKOVÁ, S.; BARTÁK, M.; SEDLÁČEK, I.

Released

9. 5. 2023

Publisher

ELSEVIER GMBH

Location

MUNICH

ISBN

0723-2020

Periodical

SYSTEMATIC AND APPLIED MICROBIOLOGY

Year of study

46

Number

4

State

Federal Republic of Germany

Pages from

1

Pages to

9

Pages count

9

URL

https://www.sciencedirect.com/science/article/pii/S0723202023000334

BibTex

@article{BUT183881,
  author="Dana {Nováková} and Vendula {Koublová} and Karel {Sedlář} and Eva {Staňková} and Stanislava {Králová} and Pavel {Švec} and Meina {Neumann-Schaal} and Jacqueline {Wolf} and Sylva {Koudelková} and Miloš {Barták} and Ivo {Sedláček}",
  title="Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica",
  journal="SYSTEMATIC AND APPLIED MICROBIOLOGY",
  year="2023",
  volume="46",
  number="4",
  pages="1--9",
  doi="10.1016/j.syapm.2023.126424",
  issn="0723-2020",
  url="https://www.sciencedirect.com/science/article/pii/S0723202023000334"
}