Publication detail

Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional "amino-glue" surfaces

BUCHTELOVÁ, M. JANŮ, L. NEČAS, D. KŘÍŽKOVÁ, P. BARTOŠÍKOVÁ, J. MEDALOVÁ, J. KOLSKÁ, Z. HEGEMANN, D. ZAJÍČKOVÁ, L.

Original Title

Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional "amino-glue" surfaces

Type

journal article in Web of Science

Language

English

Original Abstract

Plasma polymers (PPs) can easily modify material surfaces to improve their bio-applicability due to match-made surface-free energy and functionality. However, cell adhesion to PPs typically composed of various functional groups has not yet been fully understood. We explain the origin of strong resistance to trypsin treatment previously noted for nonendothelial cells on amine PPs. It is caused mainly by nonspecific adhesion of negatively charged parts of transmembrane proteins to the positively charged amine PP surface, enabled by thin glycocalyx. However, endothelial cells are bound primarily by their thick, negatively charged glycocalyx and sporadically by integrins in kinetic traps, both cleaved by trypsin. Cell scratching by atomic force microscopy tip confirmed the correlation of trypsin resistance to the strength of cell adhesion.

Keywords

amine plasma polymer; carboxyl plasma polymer; cell adhesion; glycocalyx; nonspecific adhesion

Authors

BUCHTELOVÁ, M.; JANŮ, L.; NEČAS, D.; KŘÍŽKOVÁ, P.; BARTOŠÍKOVÁ, J.; MEDALOVÁ, J.; KOLSKÁ, Z.; HEGEMANN, D.; ZAJÍČKOVÁ, L.

Released

1. 6. 2023

Publisher

Wiley-VCH

Location

WEINHEIM

ISBN

1612-8869

Periodical

Plasma Processes and Polymers

Year of study

20

Number

6

State

Federal Republic of Germany

Pages from

1

Pages to

15

Pages count

15

URL

https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157

Full text in the Digital Library

http://hdl.handle.net/11012/213691

BibTex

@article{BUT183943,
  author="Martina {Janůšová} and Lucie {Janů} and David {Nečas} and Petra {Křížková} and Jana {Bartošíková} and Jiřina {Medalová} and Zdeňka {Kolská} and Dirk {Hegemann} and Lenka {Zajíčková}",
  title="Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional {"}amino-glue{"} surfaces",
  journal="Plasma Processes and Polymers",
  year="2023",
  volume="20",
  number="6",
  pages="15",
  doi="10.1002/ppap.202200157",
  issn="1612-8869",
  url="https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157"
}