Publication detail

Genetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow

GRZESIAK, J. GAWOR, J. ROGALA, M. KOUŘILOVÁ, X. OBRUČA, S.

Original Title

Genetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow

Type

journal article in Web of Science

Language

English

Original Abstract

In recent years, extremophilic microorganisms have been employed as producers of the microbial bioplastics polyhydroxyalkanoates (PHA), which are of great biotechnological value. Nevertheless, cold-loving or psychrophilic (cryophilic) bacteria have been neglected in this regard. Here, we present an investigation of the Arctic glacier-derived PHA producer Acidovorax sp. A1169. Biolog GEN III Microplates were used as a screening tool to identify the most suitable carbon substrate concerning PHA synthesis. The strain produced homopolymer poly(3-hydroxybutyrate) (PHB) most efficiently (2 g/L) at a temperature of 15 ? when supplied with fructose or mannitol as carbon sources with a substantial decrease of PHB biosynthesis at 17.5 ?. The PHB yield did not increase considerably or even decreased when carbon source concentration exceeded 10 g/L hinting that the strain is oligotrophic in nature. The strain was also capable of introducing 3-hydroxyvalerate (3HV) into the polymer structure, which is known to improve PHA thermoplastic properties. This is the first investigation providing insight into a PHA biosynthesis process by means of a true psychrophile, offering guidelines on polar-region bacteria cultivation, production of PHA and also on the methodology for genetic engineering of psychrophiles.

Keywords

Extremophile; Arctic; Bioplastics; Low-temperature; biotechnology; Oligotrophy

Authors

GRZESIAK, J.; GAWOR, J.; ROGALA, M.; KOUŘILOVÁ, X.; OBRUČA, S.

Released

1. 12. 2023

Publisher

Springer

ISBN

1431-0651

Periodical

EXTREMOPHILES

Year of study

27

Number

3

State

Japan

Pages from

1

Pages to

14

Pages count

14

URL

https://link.springer.com/article/10.1007/s00792-023-01311-5

Full text in the Digital Library

http://hdl.handle.net/11012/245178

BibTex

@article{BUT184904,
  author="Jakub {Grzesiak} and Jan {Gawor} and Małgorzata Marta {Rogala} and Xenie {Kouřilová} and Stanislav {Obruča}",
  title="Genetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow",
  journal="EXTREMOPHILES",
  year="2023",
  volume="27",
  number="3",
  pages="1--14",
  doi="10.1007/s00792-023-01311-5",
  issn="1431-0651",
  url="https://link.springer.com/article/10.1007/s00792-023-01311-5"
}