Publication detail

Biosensor with electrochemically active nanocomposites for signal amplification and simultaneous detection of three ovarian cancer biomarkers

KOVAROVA, A. KASTRATI, G. PEKARKOVA, J. METELKA, R. DRBOHLAVOVA, J. BILKOVA, Z. SELESOVSKA, R. KORECKA, L.

Original Title

Biosensor with electrochemically active nanocomposites for signal amplification and simultaneous detection of three ovarian cancer biomarkers

Type

journal article in Web of Science

Language

English

Original Abstract

The current trend in medical diagnostic methods is to simplify and accelerate diagnosis and bring testing closer to the patient while maintaining the existing analytical criteria. Point-of-care tests (POCT) allow improved screening of population and rapid decisions regarding patient care. In case of cancer, this is a very desirable and valuable factor affecting the survival rate. Biosensors exploiting the bioaffinity interaction of target molecules with specific antibodies, combined with screen-printed electrodes as detection units, meet these criteria perfectly. In this study, we present a new sandwich-type magneto-immunosensor with electrochemical signal generating nanocomposites that was designed for the simultaneous detection and quantification of three biomarkers associated with ovarian cancer, namely human epididymis protein 4 (HE4), alpha-fetoprotein (AFP), and cancer antigen 125 (CA-125). The simultaneous detection was enabled by three different electroactive nanomaterials, namely gold nanoparticles (AuNPs), and CdTe and PbS core-type quantum dots (QDs), which were conjugated with three specific antibodies. Mesoporous silica nanoparticles (SiNPs) were incorporated in nanocomposite for higher loading of electroactive labels, leading to substantial enhancement of electrochemical signal. Square-wave anodic/cathodic stripping voltammetry was used for the detection of metal ions released quantitatively from the captured bioconjugates. Combination of these three elements provided separate non interfering signals, hence enabling simultaneous detection. Using this multiplex immunosensor, up to three biomarkers within the linear concentration ranges of 0.02-20 pM for HE4, 0.45-450 IU L 1 for CA-125, and 0.1-500 ng L 1 for AFP could be detected and quantified. The analytical parameters obtained fit the requirements of early-stage disease detection.

Keywords

Quantum dot; Multiplex detection; Stripping voltammetry; Point-of-care testing; Ovarian cancer biomarker; Silica nanoparticle

Authors

KOVAROVA, A.; KASTRATI, G.; PEKARKOVA, J.; METELKA, R.; DRBOHLAVOVA, J.; BILKOVA, Z.; SELESOVSKA, R.; KORECKA, L.

Released

20. 11. 2023

Publisher

PERGAMON-ELSEVIER SCIENCE LTD

Location

OXFORD

ISBN

0013-4686

Periodical

Electrochimica Acta

Year of study

469

Number

neuvedeno

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

9

Pages count

9

URL

https://www.sciencedirect.com/journal/electrochimica-acta/vol/469/suppl/C

BibTex

@article{BUT185499,
  author="Aneta {Kovářová} and Gylxhane {Kastrati} and Jana {Pekárková} and Radovan {Metelka} and Jana {Drbohlavová} and Zuzana {Bílková} and Renata {Šelešovská} and Lucie {Korecka}",
  title="Biosensor with electrochemically active nanocomposites for signal amplification and simultaneous detection of three ovarian cancer biomarkers",
  journal="Electrochimica Acta",
  year="2023",
  volume="469",
  number="neuvedeno",
  pages="1--9",
  doi="10.1016/j.electacta.2023.143213",
  issn="0013-4686",
  url="https://www.sciencedirect.com/journal/electrochimica-acta/vol/469/suppl/C"
}