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KOŠARIŠŤANOVÁ, L. ŘÍHÁČEK, M. SUCHÁ, F. MILOSAVLJEVIĆ, V. ŠVEC, P. DORAZILOVÁ, J. VOJTOVÁ, L. ANTAL, P. KOPEL, P. PATOČKA, Z. ADAM, V. ŽŮREK, L. DOLEŽELÍKOVÁ, K.
Original Title
Synergistic antibacterial action of the iron complex and ampicillin against Staphylococcus aureus
Type
journal article in Web of Science
Language
English
Original Abstract
Resistance to antibiotics among bacteria of clinical importance, including Staphylococcus aureus, is a serious problem worldwide and the search for alternatives is needed. Some metal complexes have antibacterial properties and when combined with antibiotics, they may increase bacterial sensitivity to antimicrobials. In this study, we synthesized the iron complex and tested it in combination with ampicillin against S. aureus. An iron complex was synthesized and characterized using spectroscopy methods. Confirmation of the synergistic effect between the iron complex (Fe16) and ampicilin (AMP) was performed using zeta-potential, infrared spectra and FICI index calculated from the minimum inhibitory concentration (MIC) from the checkerboard assay. Cytotoxic properties of combination Fe16 + AMP was evaluated on eukaryotic cell line. Impact of combination Fe16 + AMP on chosen genes of S. aureus were performed by Quantitative Real-Time PCR. The MIC of Fe16 + AMP was significantly lower than that of AMP and Fe16 alone. Furthermore, the infrared spectroscopy revealed the change in the zeta-potential of Fe16 + AMP. We demonstrated the ability of Fe16 + AMP to disrupt the bacterial membrane of S. aureus and that likely allowed for better absorption of AMP. In addition, the change in gene expression of bacterial efflux pumps at the sub-inhibitory concentration of AMP suggests an insufficient import of iron into the bacterial cell. At the same time, Fe16 + AMP did not have any cytotoxic effects on keratinocytes. Combined Fe16 + AMP therapy demonstrated significant synergistic and antimicrobial effects against S. aureus. This study supports the potential of combination therapy and further research.
Keywords
Staphylococcus aureus; Iron complex; Ampicillin; Synergy; Antimicrobial activity
Authors
KOŠARIŠŤANOVÁ, L.; ŘÍHÁČEK, M.; SUCHÁ, F.; MILOSAVLJEVIĆ, V.; ŠVEC, P.; DORAZILOVÁ, J.; VOJTOVÁ, L.; ANTAL, P.; KOPEL, P.; PATOČKA, Z.; ADAM, V.; ŽŮREK, L.; DOLEŽELÍKOVÁ, K.
Released
6. 10. 2023
Publisher
BMC
Location
LONDON
ISBN
1471-2180
Periodical
BMC MICROBIOLOGY
Year of study
23
Number
1
State
United Kingdom of Great Britain and Northern Ireland
Pages from
Pages to
8
Pages count
URL
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-03034-1
Full text in the Digital Library
http://hdl.handle.net/11012/245098
BibTex
@article{BUT185666, author="Ludmila {Košarišťanová} and Martin {Říháček} and Františka {Suchá} and Vedran {Milosavljević} and Pavel {Švec} and Jana {Matulová} and Lucy {Vojtová} and Peter {Antal} and Pavel {Kopel} and Zdeněk {Patočka} and Vojtěch {Adam} and Luděk {Žůrek} and Kristýna {Doleželíková}", title="Synergistic antibacterial action of the iron complex and ampicillin against Staphylococcus aureus", journal="BMC MICROBIOLOGY", year="2023", volume="23", number="1", pages="1--8", doi="10.1186/s12866-023-03034-1", issn="1471-2180", url="https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-03034-1" }