Publication detail

First Screening of PHA Production in Cold-Adapted Bacteria from Antarctica

ŠLOSÁROVÁ, K. BYRTUSOVÁ, D. PERNICOVÁ, I. AKULAVA, V. SMIRNOVA, M. SLANINOVÁ, E. OBRUČA, S. SHAPAVAL, V.

Original Title

First Screening of PHA Production in Cold-Adapted Bacteria from Antarctica

Type

abstract

Language

English

Original Abstract

Some bacteria naturally produce polyhydroxyalkanoates (PHA) in order to intracellularly store carbon and energy. From a technological point of view, PHA have great potential as biodegradable versions of conventional plastics. However, their main disadvantage compared to them is the high cost of production. Therefore, the research is focusing on ways to overcome this obstacle, by screening many bacterial strains that can survive extreme conditions. The main advantage that extremophiles bring from the perspective of biotechnology is a reduction in the cost of sterility of the work. Additionally, there is limited research devoted to psychrophiles, microorganisms living in cold areas. The results of the screening involving seventy-eight cold-adapted bacterial strains isolated from East Antarctica are presented in this work. Both genotypic and phenotypic methods were used for the selection of potential PHA producers. The polymerase chain reaction demonstrated the presence of genes encoding PHA synthases. From the phenotypic testing, staining with Nile Red and Sudan Black B, FTIR spectroscopy in combination with a high-throughput microculture system (Duetz-MTPS), and gas chromatography were used to explore the bacterial biomass for the presence of PHA. Based on the results the highest biomass production and PHA accumulation (0.6 g/L) showed Pseudomonas sp. belonging to the green snow isolates. Interestingly, a high synthesis of PHA, specifically P(3HB), was observed on complex BHI medium with extra glucose addition, which did not provide the nitrogen limitation that is typical for the stimulation of the PHA accumulation. It can be assessed that the presence of promising producers among polar bacteria was confirmed by PCR, FTIR, and GC, and the yield of PHA can be further increased by optimizing the media composition and growth conditions.

Keywords

psychrophilic bacteria, polyhydroxyalkanoates, high-throughput screening

Authors

ŠLOSÁROVÁ, K.; BYRTUSOVÁ, D.; PERNICOVÁ, I.; AKULAVA, V.; SMIRNOVA, M.; SLANINOVÁ, E.; OBRUČA, S.; SHAPAVAL, V.

Released

30. 11. 2023

ISBN

978-80-214-6204-5

Book

Sborník abstraktů

Edition number

první

Pages from

70

Pages to

70

Pages count

1

BibTex

@misc{BUT185692,
  author="ŠLOSÁROVÁ, K. and BYRTUSOVÁ, D. and PERNICOVÁ, I. and AKULAVA, V. and SMIRNOVA, M. and SLANINOVÁ, E. and OBRUČA, S. and SHAPAVAL, V.",
  title="First Screening of PHA Production in Cold-Adapted Bacteria from Antarctica",
  booktitle="Sborník abstraktů",
  year="2023",
  edition="první",
  pages="70--70",
  isbn="978-80-214-6204-5",
  note="abstract"
}