Publication detail

Detection of Bioindicator Elements in Tissues: Case of Human Teeth Ankylosis

KONEČNÁ, A. POŘÍZKA, P. ŠTEMBÍREK, J. HURNÍK, P. VRLÍKOVÁ, L. BUCHTOVÁ, M. KAISER, J.

Original Title

Detection of Bioindicator Elements in Tissues: Case of Human Teeth Ankylosis

Type

abstract

Language

English

Original Abstract

Ankylosis is a pathological condition that results in a firm fusion of tooth cementum and surrounding alveolar bone. Possible causes of ankylosis are still unclear, but one hypothesis states that ankylosis develops as a result of damage in the cementum layer, followed by bone cells binding in that area. Thus, this study focused on the analysis of the elemental changes in the ankylotic area as well as in the surrounding tissues. Elemental changes were studied by Laser-Induced Breakdown Spectroscopy (LIBS) which is an analytical method based on optical emission spectroscopy used for determining the elemental composition of examined samples. Thanks to its possibility of simultaneous multi-elemental and spatially resolved analysis, LIBS is mainly used for fast semi-quantitative analyses, in biological applications also known as bioimaging. Bioimaging results in detailed 2D distributional maps for each detected element. Each map depicts elemental distribution as well as the change in the elemental concentration, which makes it possible to distinguish, for example, differences between a healthy and a diseased part of tissue. In this work, the characterization of human teeth ankylosis was performed by LIBS imaging with a spatial resolution of 20 µm. Results showed an elemental disbalance in the ankylotic area – a significant increase in calcium, magnesium, and phosphorus levels confirming the fusion of cementum and alveolar bone.

Keywords

LIBS, bioimaging, ankylosis, human teeth

Authors

KONEČNÁ, A.; POŘÍZKA, P.; ŠTEMBÍREK, J.; HURNÍK, P.; VRLÍKOVÁ, L.; BUCHTOVÁ, M.; KAISER, J.

Released

10. 10. 2023

BibTex

@misc{BUT186857,
  author="Anna {Faruzelová} and Pavel {Pořízka} and Jan {Štembírek} and Pavel {Hurník} and Lucie {Vrlíková} and Marcela {Buchtová} and Jozef {Kaiser}",
  title="Detection of Bioindicator Elements in Tissues: Case of Human Teeth Ankylosis",
  year="2023",
  note="abstract"
}