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JYOTI, J. MUÑOZ MARTIN, J. PUMERA, M.
Original Title
Quantum Material-Based Self-Propelled Microrobots for the Optical "On-the-Fly" Monitoring of DNA
Type
journal article in Web of Science
Language
English
Original Abstract
Quantum dot-based materials have been found to be excellent platforms for biosensing and bioimaging applications. Herein, self-propelled microrobots made of graphene quantum dots (GQD-MRs) have been synthesized and explored as unconventional dynamic biocarriers toward the optical "on-the-fly" monitoring of DNA. As a first demonstration of applicability, GQD-MRs have been first biofunctionalized with a DNA biomarker (i.e., fluorescein amidite-labeled, FAM-L) via hydrophobic pi-stacking interactions and subsequently exposed toward different concentrations of a DNA target. The biomarker-target hybridization process leads to a biomarker release from the GQD-MR surface, resulting in a linear alteration in the fluorescence intensity of the dynamic biocarrier at the nM range (1-100 nM, R-2 = 0.99), also demonstrating excellent selectivity and sensitivity, with a detection limit as low as 0.05 nM. Consequently, the developed dynamic biocarriers, which combine the appealing features of GQDs (e.g., water solubility, fluorescent activity, and supramolecular pi-stacking interactions) with the autonomous mobility of MRs, present themselves as potential autonomous micromachines to be exploited as highly efficient and sensitive "on-the-fly" biosensing systems. This method is general and can be simply customized by tailoring the biomarker anchored to the GQD-MR's surface.
Keywords
microrockets; fluorescence; self-propelledmicromotors; DNA biosensor; FRET
Authors
JYOTI, J.; MUÑOZ MARTIN, J.; PUMERA, M.
Released
11. 12. 2023
Publisher
AMER CHEMICAL SOC
Location
WASHINGTON
ISBN
1944-8252
Periodical
ACS applied materials & interfaces
Year of study
15
Number
50
State
United States of America
Pages from
58548
Pages to
58555
Pages count
8
URL
https://pubs.acs.org/doi/10.1021/acsami.3c09920
Full text in the Digital Library
http://hdl.handle.net/11012/245192
BibTex
@article{BUT186982, author="Jyoti {Jyoti} and Jose Maria {Muñoz Martin} and Martin {Pumera}", title="Quantum Material-Based Self-Propelled Microrobots for the Optical {"}On-the-Fly{"} Monitoring of DNA", journal="ACS applied materials & interfaces", year="2023", volume="15", number="50", pages="58548--58555", doi="10.1021/acsami.3c09920", issn="1944-8252", url="https://pubs.acs.org/doi/10.1021/acsami.3c09920" }