Publication detail

Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings

BAISHYA, K. VRCHOVECKÁ, K. ALIJANI, M. RODRIGUEZ PEREIRA, J. THALLURI, S. PÁVKOVÁ GOLDBERGOVÁ, M. PŘIBYL, J. MACÁK, J.

Original Title

Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings

Type

journal article in Web of Science

Language

English

Original Abstract

The present work studies anodic TiO2 nanotube (TNT) layers and their surface modifications for enhancing the cell behavior of human gingival fibroblast cells (hGFs) with the contribution of bio-AFM (Atomic Force Micro-scopy) method. TNT layers, prepared via electrochemical anodization of Ti, with an average tube diameter of 15, 30, and 100 nm, were used as primary substrates for the study. Flat Ti foils were used as reference substrates. Part of the substrates was coated by ultrathin TiO2 coatings (approximate to 0.3 nm thin) using Atomic Layer Deposition (ALD). The cell growth and adhesion of hGFs on the TNT layers and Ti foils were compared between ALD coated and uncoated ones. The supplemental coatings altered the surface chemistry of the TNT layers, particularly shading the fluorine and carbon impurities within anodic TiO2, while preserving the original structure and morphology. The presented approach of very mild surface modification remarkably effects the material's biocompatibility and possess great prospect as implant materials. For the first time, the TNT/cell interface was investigated using bio-AFM in terms of Young's modulus, stiffness, cell adhesive force and roughness. Improved biocompatibility was studied in terms of increased cell viability, density, cell cytoskeleton orientation and overall stiffness of the hGFs.

Keywords

Nanotube; hGFs; Ti foils; Atomic layer deposition; Bio-AFM

Authors

BAISHYA, K.; VRCHOVECKÁ, K.; ALIJANI, M.; RODRIGUEZ PEREIRA, J.; THALLURI, S.; PÁVKOVÁ GOLDBERGOVÁ, M.; PŘIBYL, J.; MACÁK, J.

Released

1. 12. 2023

Publisher

ELSEVIER

Location

AMSTERDAM

ISBN

2666-5239

Periodical

Applied Surface Science Advances

Year of study

18

Number

1

State

Kingdom of the Netherlands

Pages count

12

URL

https://www.sciencedirect.com/science/article/pii/S2666523923000934?via%3Dihub

Full text in the Digital Library

http://hdl.handle.net/11012/245177

BibTex

@article{BUT187353,
  author="Kaushik {Baishya} and Katarina {Vrchovecká} and Mahnaz {Alijani} and Jhonatan {Rodriguez Pereira} and Sitaramanjaneya Mouli {Thalluri} and Monika {Pávková Goldbergová} and Jan {Přibyl} and Jan {Macák}",
  title="Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings",
  journal="Applied Surface Science Advances",
  year="2023",
  volume="18",
  number="1",
  pages="12",
  doi="10.1016/j.apsadv.2023.100459",
  issn="2666-5239",
  url="https://www.sciencedirect.com/science/article/pii/S2666523923000934?via%3Dihub"
}