Publication detail

Bioprospecting of biosurfactant-producing bacteria for hydrocarbon bioremediation: Optimization and characterization

Bellebcir, Anfal Merouane, Fateh Chekroud, Karim Bounabi, Hadjira Vasseghian, Yasser Kamyab, Hesam Chelliapan, Shreeshivadasan Klemes, Jiri Jaromir Berkani, Mohammed

Original Title

Bioprospecting of biosurfactant-producing bacteria for hydrocarbon bioremediation: Optimization and characterization

Type

journal article in Web of Science

Language

English

Original Abstract

Biosurfactants have been found capable of replacing synthetic surfactants which include ongoing bioprospecting of biosurfactant-producing bacteria as well as process optimization for maximum biosurfactant production. In this study, five morphologically distinct actinomycete strains isolated from hydrocarbon-polluted soil collected from an oil spill surface in Southeastern Algeria were tested for their ability to produce biosurfactants using preliminary biosurfactant screening assays. The 7SDS strain was selected as the most promising biosurfactant producer due to its greatest oil displacement diameter (7.83 & PLUSMN;0.15 cm), emulsification index (59.66 & PLUSMN;0.44%), and enhanced surface tension reduction (30.04 & PLUSMN;0.51 mN/m); it was identified as Streptomyces thinghirensis 7SDS using 16S rDNA sequence analysis. The 7SDS strain's biosurfactant production was optimized using the Face-centered central composite design (CCD) based on response surface methodology (RSM). To this end, five independent factors, i.e., residual frying oil, used engine oil, whey, CS filtrate, and incubation time, were assessed. The RSM's model predicted a surface tension of 27.48 mN/m using 2.44% (v/v) residual frying oil, 0.35% (v/v) used motor oil, 0.83% (v/v) whey, 0.39% (v/v) CS filtrate, and an incubation time of 219.3 h. The optimized medium produced 8.79 g/L of biosurfactant. The produced biosurfactant allows one to reduce the surface tension of distilled water from 70.86 mN/m to 27.96 mN/m at a critical micelle concentration of 350 mg/L, even over a wide range of pH (2.0-12.0), temperature (4-120 & DEG;C), and salinity (2-12%, W/V). Biochemical (Biuret, phenol-sulfuric acid, and phosphate tests) and compositional (FTIR and GC-MS) characterizations confirmed the phospholipid nature of the produced biosurfactant. Interestingly, the produced BS demonstrated significant antimicrobial activity as well as intriguing activity in removing hydrocarbons from polluted soil. Because of their appealing biological properties, strain 7SDS and its biosurfactant are attractive targets for a variety of applications such as biomedicine and environmental ones.

Keywords

Biosurfactant; Phospholipid; Response Surface Methodology; Streptomyces

Authors

Bellebcir, Anfal; Merouane, Fateh; Chekroud, Karim; Bounabi, Hadjira; Vasseghian, Yasser; Kamyab, Hesam; Chelliapan, Shreeshivadasan; Klemes, Jiri Jaromir; Berkani, Mohammed

Released

1. 10. 2023

Publisher

KOREAN INSTITUTE CHEMICAL ENGINEERSF.5, 119, ANAM-RO, SEONGBUK-GU, SEOUL 136-075, SOUTH KOREA

Location

KOREAN INSTITUTE CHEMICAL ENGINEERSF.5, 119, ANAM-RO, SEONGBUK-GU, SEOUL 136-075, SOUTH KOREA

ISBN

0256-1115

Periodical

KOREAN JOURNAL OF CHEMICAL ENGINEERING

Year of study

10

Number

40

State

Republic of Korea

Pages from

2497

Pages to

2512

Pages count

16

URL

https://link.springer.com/article/10.1007/s11814-023-1418-y

BibTex

@article{BUT187519,
  author="Bellebcir, Anfal and Merouane, Fateh and Chekroud, Karim and Bounabi, Hadjira and Vasseghian, Yasser and Kamyab, Hesam and Chelliapan, Shreeshivadasan and Klemes, Jiri Jaromir and Berkani, Mohammed",
  title="Bioprospecting of biosurfactant-producing bacteria for hydrocarbon bioremediation: Optimization and characterization",
  journal="KOREAN JOURNAL OF CHEMICAL ENGINEERING",
  year="2023",
  volume="10",
  number="40",
  pages="2497--2512",
  doi="10.1007/s11814-023-1418-y",
  issn="0256-1115",
  url="https://link.springer.com/article/10.1007/s11814-023-1418-y"
}