Lyophilisation as a useful method of carrier system preparation

article in a collection out of WoS and Scopus

English

The aim of this study was to prepare native hyaluronan-based carrier systems using lyophilisation as a suitable method to ensure an interaction between certain drugs and the polymeric structure which would lead to an increased solubility of hydrophobic drugs. For the preparation of such a system, the tert-butyl alcohol was used as a co-solvent with the ability to support the hydrophobic effect between the drug and a hydrophobic domain of hyaluronan. Previous studies have confirmed that freeze-drying is a useful method for carrier system preparation. However, this study focuses on the preparation of such systems with samples of real drugs, which were previously replaced by fluorescence sondes. Since all drug samples have good fluorescence characteristics, fluorescence spectroscopy was used as the main method to determine the success of the preparation. Other methods included a thermogravimetric analysis and the Fourier-transform infrared spectroscopy. All the mentioned methods have confirmed that lyophilisation is a useful method for the preparation of the carrier systems with real drugs. Great results have been achieved in terms of the solubility of freeze-dried systems. In the last step, the co-solvent residues were determined by head-space solid phase microextraction. This method also provided some valuable information as well as gave the direction for future research. Freeze-drying is a gentle technique for drying samples that could be degraded by high temperatures. We are talking primarily about biological samples such as proteins, enzymes, saccharides etc. During the process of freeze-drying, samples in vacuum are frozen. As the temperature is then slowly raised, the solvent sublimes from the sample. The drying is divided into two parts; during primary drying nearly 95 % of water sublime, therefore the aim of secondary drying is to get rid of water residues, which are the molecules of water attached to the sample surface by adsorption. Once secondary drying is complete, the samples are fragile but retain their biological function.

hyaluronan, freeze-drying, drug carrier systems, fluorescence spectroscopy

ŘIHÁK, M.; ENEV, V.; VENEROVÁ, T.

30. 11. 2023

FCH VUT v Brně

Brno

978-80-214-6204-5

Chemie je život 2023 - Sborník příspěvků

VUT

první

38

47

10