Publication detail

Multi-wavelength deep-ultraviolet absorbance detector based upon program-controlled pulsing light-emitting diodes

MIKHAIL, I.E. HEMIDA, M. LEBANOV, L. ASTRAKHANTSEVA, S. GUPTA, V. HORTIN, P. PARRY, J.S. MACKA, M. PAULL, B.

Original Title

Multi-wavelength deep-ultraviolet absorbance detector based upon program-controlled pulsing light-emitting diodes

Type

journal article in Web of Science

Language

English

Original Abstract

A novel approach for multi-wavelength ultraviolet (UV) absorbance detection has been introduced employing a single board computer (SBC) with a field programmable gate array (FPGA), Red Pitaya SBC, to generate separated micro pulses for three deep-ultraviolet light-emitting diodes (DUV-LEDs), lambda max = 235, 250, and 280 nm, along with data acquisition and processing via a custom-made program. The pulse set generation and data acquisition were synchronized using the SBC. The outputs of the three pulsing DUV-LEDs were combined and transmitted to the flow cell via a solarisation resistant trifurcated optical fiber (OF). An ultra-fast responding photodiode was connected to the optical-fiber-compatible flow cell to record the intensity of the DUV pulses. Upper limit of detector linearity (A95 %) was found to be 1917 mAU, 2189 mAU, and 1768 mAU at 235 nm, 250 nm, and 280 nm, respectively, with stray light <= 0.9 %. In addition, the effective path length (Leff) was estimated to be >= 98.0 % of the length of the used flow cell (50 mm). The new pulsed multi-LEDs absorbance detector (PMLAD) has been successfully coupled with a standard liquid chromatograph and utilized for the analysis of pharmaceuticals. Paracetamol, caffeine, and aspirin were simultaneously determined at 250, 280, and 235 nm, respectively, using the PMLAD. The absorbance ratios between the different wavelengths were applied to further confirm the identity of the studied compounds. Excellent linearity was achieved over a range of 0.1-3.2 mu g/mL for paracetamol, 0.4-6.4 mu g/mL for caffeine, and 0.8-12.8 mu g/mL for aspirin with a regression correlation coefficient (r2) >= 0.99996. The quantitation limits (LOQs) were 0.10 mu g/mL, 0.38 mu g/mL, and 0.66 mu g/mL for paracetamol, caffeine, and aspirin, respectively.

Keywords

Multi-wavelength detection; Pulsing light-emitting diodes; Deep-ultraviolet absorption; High performance liquid chromatography; Red Pitaya

Authors

MIKHAIL, I.E.; HEMIDA, M.; LEBANOV, L.; ASTRAKHANTSEVA, S.; GUPTA, V.; HORTIN, P.; PARRY, J.S.; MACKA, M.; PAULL, B.

Released

25. 10. 2023

Publisher

ELSEVIER

Location

AMSTERDAM

ISBN

1873-3778

Periodical

JOURNAL OF CHROMATOGRAPHY A

Year of study

1709

Number

464382

State

Kingdom of the Netherlands

Pages count

9

URL

https://www.sciencedirect.com/science/article/pii/S0021967323006076?via%3Dihub

BibTex

@article{BUT187783,
  author="MIKHAIL, I.E. and HEMIDA, M. and LEBANOV, L. and ASTRAKHANTSEVA, S. and GUPTA, V. and HORTIN, P. and PARRY, J.S. and MACKA, M. and PAULL, B.",
  title="Multi-wavelength deep-ultraviolet absorbance detector based upon program-controlled pulsing light-emitting diodes",
  journal="JOURNAL OF CHROMATOGRAPHY A",
  year="2023",
  volume="1709",
  number="464382",
  pages="9",
  doi="10.1016/j.chroma.2023.464382",
  issn="1873-3778",
  url="https://www.sciencedirect.com/science/article/pii/S0021967323006076?via%3Dihub"
}