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ZHANG, H. CAO, L. BRODSKÝ, J. GABLECH, I. XU, F. LI, Z. KORABEČNÁ, M. NEUŽIL, P.
Original Title
Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications
Type
journal article in Web of Science
Language
English
Original Abstract
This paper offers an exhaustive comparison of quantitative PCR (qPCR) and digital PCR (dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from pathogen detection, and cancer screening to environment monitoring. The manuscript outlines the foundational theories behind both technologies and scrutinizes their capabilities, advances, and limits. It deeply investigates the primary factors based on the sample's nature, sample preparation strategies, and employing key parameters like reliability, accuracy, and specificity, thus critically evaluating the merits and demerits of each technology. It also proposes the avenues of research and development that impact both technologies. The paper concludes that the choice between qPCR and dPCR depends on factors such as the nature of the sample, the purpose of the study, and practical considerations like cost and operational ease. Our overarching goal is to furnish a guide that aids researchers in selecting the optimal PCR-based technique for their assay development.
Keywords
qPCR; dPCR
Authors
ZHANG, H.; CAO, L.; BRODSKÝ, J.; GABLECH, I.; XU, F.; LI, Z.; KORABEČNÁ, M.; NEUŽIL, P.
Released
1. 5. 2024
Publisher
Elsevier
Location
UK
ISBN
0165-9936
Periodical
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
Year of study
174
Number
5
State
United Kingdom of Great Britain and Northern Ireland
Pages count
17
URL
https://www.sciencedirect.com/science/article/pii/S0165993624001584
BibTex
@article{BUT188407, author="ZHANG, H. and CAO, L. and BRODSKÝ, J. and GABLECH, I. and XU, F. and LI, Z. and KORABEČNÁ, M. and NEUŽIL, P.", title="Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications", journal="TRAC-TRENDS IN ANALYTICAL CHEMISTRY", year="2024", volume="174", number="5", pages="17", doi="10.1016/j.trac.2024.117676", issn="0165-9936", url="https://www.sciencedirect.com/science/article/pii/S0165993624001584" }