Publication detail

Synthetic auxin herbicide 2,4-D and its influence on a model BY-2 suspension

MUSELÍKOVÁ, K., MOURALOVÁ, K.

Original Title

Synthetic auxin herbicide 2,4-D and its influence on a model BY-2 suspension

Type

journal article in Web of Science

Language

English

Original Abstract

2,4-D is a broadly used auxin herbicide. The presence of the 2,4-D synthetic auxin in the medium is imperative for long-term BY-2 tobacco suspension viability. The precise mechanism of this symbiosis of the suspension and the synthetic auxin remains unclear. Our goal was to study the hormonal regulation of the growth of the cell suspension; and to describe the experiments clarifying the interaction between the chosen growth regulators and phytohormones on the cellular level, specifically between the 2,4-D synthetic auxin and the native stress phytohormone — ethylene. This study examined the influence of low 2,4-D concentrations stimulating cell growth in vitro as well as the influence of high herbicide concentrations on the model tobacco BY-2 suspension. The culture took 6 days. Different parameters were evaluated, including the influence of different 2,4-D concentrations on the production of the phytohormone ethylene and its precursor 1-Aminocyclopropane-1-carboxylic acid (ACC) in the tobacco cells. The content of 2,4-D in the cells and the medium was established. The observations of the morphological changes showed that a heavy impregnation of the cell walls taking place depending on the concentration of 2,4-D. A dramatic increase in protective polysaccharides and a remodulation of the cell walls by the formation of a pectin shield in artificial conditions were expected and observed. At the same time, massive production of the stress phytohormone ethylene took place, and, because of that, plant mutagenicity, anomalous tumour-type proliferation growth, and the production of supercells were observed. The hypothesis of the protective shield is discussed.

Keywords

BY-2 cell suspension; 2,4-dichlorfenoxyacetic acid; 2,4-D; ethylene; plant mutagenicity; pectin shield

Authors

MUSELÍKOVÁ, K., MOURALOVÁ, K.

Released

14. 4. 2024

Publisher

Springer

ISBN

0301-4851

Periodical

MOLECULAR BIOLOGY REPORTS

Year of study

51

Number

1

State

Kingdom of the Netherlands

Pages count

14

URL

https://link.springer.com/article/10.1007/s11033-024-09392-x

BibTex

@article{BUT188409,
  author="Kateřina {Mouralová}",
  title="Synthetic auxin herbicide 2,4-D and its influence on a model BY-2 suspension
  	",
  journal="MOLECULAR BIOLOGY REPORTS",
  year="2024",
  volume="51",
  number="1",
  pages="14",
  doi="10.1007/s11033-024-09392-x",
  issn="0301-4851",
  url="https://link.springer.com/article/10.1007/s11033-024-09392-x"
}