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OLIVER URRUTIA, C. DIEZ-ESCUDERO, A. KOLEDOVÁ, Z. ČELKO, L. HAILER, N. P. MONTUFAR JIMENEZ, E.
Original Title
Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds
Type
book chapter
Language
English
Original Abstract
In vitro cell cultures are a very useful tool for the validation of biomaterial cytocompatibility, especially for bone tissue engineering scaffolds and bone implants. In this chapter, a protocol for a static three-dimensional osteoblast cell culture on titanium scaffolds and subsequent analysis of osteogenic capacity is presented. The protocol is explained for additively manufactured titanium scaffolds, but it can be extrapolated to other scaffolds with similar size and structure, while differing in composition or manufactured technology. Additionally, the protocol can be used for culture of other adherent cell types beyond osteoblast cells such as mesenchymal stem cells.
Keywords
cell cultures; In vitro; cytocompatibility; titanium scaffolds
Authors
OLIVER URRUTIA, C.; DIEZ-ESCUDERO, A.; KOLEDOVÁ, Z.; ČELKO, L.; HAILER, N. P.; MONTUFAR JIMENEZ, E.
Released
24. 2. 2024
Publisher
Humana
Location
New York, NY
ISBN
978-1-0716-3673-2
Book
Methods in Molecular Biology
Edition
2764
Pages from
43
Pages to
60
Pages count
17
URL
https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_5#citeas
BibTex
@inbook{BUT188701, author="OLIVER URRUTIA, C. and DIEZ-ESCUDERO, A. and KOLEDOVÁ, Z. and ČELKO, L. and HAILER, N. P. and MONTUFAR JIMENEZ, E.", title="Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds", booktitle="Methods in Molecular Biology", year="2024", publisher="Humana", address="New York, NY", series="2764", pages="43--60", doi="10.1007/978-1-0716-3674-9\{_}5", isbn="978-1-0716-3673-2", url="https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_5#citeas" }