Publication detail

Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles

FARKA, Z. VYTISKOVÁ, K. MAKHNEVA, E. ZIKMUNDOVÁ, E. HOLUB, D. BUDAY, J. PROCHAZKA, D. NOVOTNÝ, K. SKLÁDAL, P. POŘÍZKA, P. KAISER, J.

Original Title

Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles

Type

journal article in Web of Science

Language

English

Original Abstract

Background: Laser-induced breakdown spectroscopy (LIBS) is a well-recognized analytical technique used for elemental analysis. This method is gaining considerable attention also in biological applications thanks to its ability for spatial mapping and elemental imaging. The implementation of LIBS in the biomedical field is based on the detection of metals or other elements that either naturally occur in the samples or are present artificially. The artificial implementation of nanoparticle labels (Tag-LIBS) enables the use of LIBS as a readout technique for immunochemical assays. However, one of the biggest challenges for LIBS to meet immunoassay readout standards is its sensitivity. Results: This paper focuses on the improvement of LIBS sensitivity for the readout of nanoparticle-based immunoassays. First, the LIBS setup was optimized on photon-upconversion nanoparticle (UCNP) droplets deposited on the microtiter plate wells. Two collection optics systems were compared, with single pulse (SP) and collinear double pulse (DP) LIBS arrangements. By deploying the second laser pulse, the sensitivity was improved up to 30 times. The optimized SP and DP setups were then employed for the indirect detection of human serum albumin based on immunoassay with UCNP-based labels. Compared to our previous LIBS study, the detection limit was enhanced by two orders of magnitude, from 10 ng mL-1 to 0.29 ng mL-1. In addition, two other immunochemical methods were used for reference, based on the readout of upconversion luminescence of UCNPs and absorbance measurement with enzyme labels. Finally, the selectivity of the assay was tested and the practical potential of Tag-LIBS was demonstrated by the successful analysis of urine samples. Significance and novelty: In this work, we improved the sensitivity of the Tag-LIBS method by combining new labels based on UCNPs with the improved collection optics and collinear DP configuration. In the instrumental setup optimization, the DP LIBS showed better sensitivity and signal-to-noise ratio than SP. The optimizations allowed the LIBS readout to surpass the sensitivity of enzyme immunoassay, approaching the qualities of upconversion luminescence readout, which is nowadays a state-of-the-art readout technique.

Keywords

Laser -induced breakdown spectroscopy; Double pulse; Tag-LIBS; Photon-upconversion nanoparticle; Immunoassay; Human serum albumin

Authors

FARKA, Z.; VYTISKOVÁ, K.; MAKHNEVA, E.; ZIKMUNDOVÁ, E.; HOLUB, D.; BUDAY, J.; PROCHAZKA, D.; NOVOTNÝ, K.; SKLÁDAL, P.; POŘÍZKA, P.; KAISER, J.

Released

22. 4. 2024

Publisher

ELSEVIER

Location

AMSTERDAM

ISBN

1873-4324

Periodical

Analytica Chimica Acta

Year of study

1299

Number

342418

State

Kingdom of the Netherlands

Pages count

8

URL

https://www.sciencedirect.com/science/article/pii/S0003267024002198?via%3Dihub

BibTex

@article{BUT188854,
  author="Zdeněk {Farka} and Karolína {Vytisková} and Ekaterina {Makhneva} and Eva {Zikmundová} and Daniel {Holub} and Jakub {Buday} and David {Prochazka} and Karel {Novotný} and Petr {Skládal} and Pavel {Pořízka} and Jozef {Kaiser}",
  title="Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles",
  journal="Analytica Chimica Acta",
  year="2024",
  volume="1299",
  number="342418",
  pages="8",
  doi="10.1016/j.aca.2024.342418",
  issn="1873-4324",
  url="https://www.sciencedirect.com/science/article/pii/S0003267024002198?via%3Dihub"
}