Publication detail

Ch(O)P-CT: quantitative morphometrical analysis of the Hindbrain Choroid Plexus by X-ray micro-computed tomography

PAROBKOVÁ, V. KOMPANÍKOVÁ, P. LÁZŇOVSKÝ, J. KAVKOVÁ, M. HAMPL, M. BUCHTOVÁ, M. ZIKMUND, T. KAISER, J. BRYJA, V.

Original Title

Ch(O)P-CT: quantitative morphometrical analysis of the Hindbrain Choroid Plexus by X-ray micro-computed tomography

Type

journal article in Web of Science

Language

English

Original Abstract

The Hindbrain Choroid Plexus is a complex, cerebrospinal fluid-secreting tissue that projects into the 4th vertebrate brain ventricle. Despite its irreplaceability in the development and homeostasis of the entire central nervous system, the research of Hindbrain Choroid Plexus and other Choroid Plexuses has been neglected by neuroscientists for decades. One of the obstacles is the lack of tools that describe the complex shape of the Hindbrain Choroid Plexus in the context of brain ventricles. Here we introduce an effective tool, termed Ch(O)P-CT, for the noninvasive, X-ray micro-computed tomography-based, three-dimensional visualization and subsequent quantitative spatial morphological analysis of developing mouse Hindbrain Choroid Plexus. Ch(O)P-CT can reliably quantify Hindbrain Choroid Plexus volume, surface area, length, outgrowth angle, the proportion of the ventricular space occupied, asymmetries and general shape alterations in mouse embryos from embryonic day 13.5 onwards. We provide evidence that Ch(O)P-CT is suitable for the unbiased evaluation and detection of the Hindbrain Choroid Plexus alterations within various mutant embryos. We believe, that thanks to its versatility, quantitative nature and the possibility of automation, Ch(O)P-CT will facilitate the analysis of the Hindbrain Choroid Plexus in the mouse models. This will ultimately accelerate the screening of the candidate genes and mechanisms involved in the onset of various Hindbrain Choroid Plexus-related diseases.

Keywords

Hindbrain choroid plexus; X-ray micro-computed tomography; 3D visualization; Morphometrics

Authors

PAROBKOVÁ, V.; KOMPANÍKOVÁ, P.; LÁZŇOVSKÝ, J.; KAVKOVÁ, M.; HAMPL, M.; BUCHTOVÁ, M.; ZIKMUND, T.; KAISER, J.; BRYJA, V.

Released

24. 1. 2024

Publisher

BMC

Location

LONDON

ISBN

2045-8118

Periodical

Fluids and Barriers of the CNS

Year of study

21

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages count

18

URL

https://fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-023-00502-8

Full text in the Digital Library

http://hdl.handle.net/11012/249332

BibTex

@article{BUT188889,
  author="Viktória {Parobková} and Petra {Kompaníková} and Jakub {Lázňovský} and Michaela {Kavková} and Marek {Hampl} and Marcela {Buchtová} and Tomáš {Zikmund} and Jozef {Kaiser} and Vítězslav {Bryja}",
  title="Ch(O)P-CT: quantitative morphometrical analysis of the Hindbrain Choroid Plexus by X-ray micro-computed tomography",
  journal="Fluids and Barriers of the CNS",
  year="2024",
  volume="21",
  number="1",
  pages="18",
  doi="10.1186/s12987-023-00502-8",
  issn="2045-8118",
  url="https://fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-023-00502-8"
}