Přístupnostní navigace
E-application
Search Search Close
Publication detail
JUREČKOVÁ, K. NYKRÝNOVÁ, M. SLANINOVÁ, E. FLEURIOT-BLITMAN, H. AMSTUTZ, V. HEŘMÁNKOVÁ, K. BEZDÍČEK, M. MRÁZOVÁ, K. HRUBANOVÁ, K. ZINN, M. OBRUČA, S. SEDLÁŘ, K.
Original Title
Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum
Type
journal article in Web of Science
Language
English
Original Abstract
Purple photosynthetic bacteria (PPB) are versatile microorganisms capable of producing various value-added chemicals, e.g., biopolymers and biofuels. They employ diverse metabolic pathways, allowing them to adapt to various growth conditions and even extreme environments. Thus, they are ideal organisms for the Next Generation Industrial Biotechnology concept of reducing the risk of contamination by using naturally robust extremophiles. Unfortunately, the potential of PPB for use in biotechnology is hampered by missing knowledge on regulations of their metabolism. Although Rhodospirillum rubrum represents a model purple bacterium studied for polyhydroxyalkanoate and hydrogen production, light/chemical energy conversion, and nitrogen fixation, little is known regarding the regulation of its metabolism at the transcriptomic level. Using RNA sequencing, we compared gene expression during the cultivation utilizing fructose and acetate as substrates in case of the wild-type strain R. rubrum DSM 467T and its knock-out mutant strain that is missing two polyhydroxyalkanoate synthases PhaC1 and PhaC2. During this first genome-wide expression study of R. rubrum, we were able to characterize cultivation-driven transcriptomic changes and to annotate non-coding elements as small RNAs.
Keywords
Rhodospirillum rubrum; RNA-Seq; Transcriptome; Genome; Depolymerase knock-out; Gene ontology; Metabolism; Fructose; Acetate; Polyhydroxyalkanoates
Authors
JUREČKOVÁ, K.; NYKRÝNOVÁ, M.; SLANINOVÁ, E.; FLEURIOT-BLITMAN, H.; AMSTUTZ, V.; HEŘMÁNKOVÁ, K.; BEZDÍČEK, M.; MRÁZOVÁ, K.; HRUBANOVÁ, K.; ZINN, M.; OBRUČA, S.; SEDLÁŘ, K.
Released
26. 6. 2024
Publisher
Elsevier
ISBN
2001-0370
Periodical
Computational and Structural Biotechnology Journal
Year of study
23
Number
December 2024
State
Kingdom of Sweden
Pages from
2681
Pages to
2694
Pages count
14
URL
https://www.sciencedirect.com/science/article/pii/S2001037024002204
Full text in the Digital Library
http://hdl.handle.net/11012/249379
BibTex
@article{BUT188890, author="Kateřina {Šabatová} and Markéta {Jakubíčková} and Eva {Slaninová} and Hugo {Fleuriot-Blitman} and Véronique {Amstutz} and Kristýna {Heřmánková} and Matěj {Bezdíček} and Kateřina {Mrázová} and Kamila {Hrubanová} and Manfred {Zinn} and Stanislav {Obruča} and Karel {Sedlář}", title="Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum", journal="Computational and Structural Biotechnology Journal", year="2024", volume="23", number="December 2024", pages="2681--2694", doi="10.1016/j.csbj.2024.06.023", issn="2001-0370", url="https://www.sciencedirect.com/science/article/pii/S2001037024002204" }