Publication detail

Multifunctional graphene quantum dots: A therapeutic strategy for neurodegenerative diseases by regulating calcium influx, crossing the blood-brain barrier and inhibiting Aβ-protein aggregation

GOMEZ PEREZ, I. KŘÍŽKOVÁ, P. DOLEČKOVÁ, A. CARDO, L. WETZL, C. PIZÚROVÁ, N. PRATO, M. MEDALOVÁ, J. ZAJÍČKOVÁ, L.

Original Title

Multifunctional graphene quantum dots: A therapeutic strategy for neurodegenerative diseases by regulating calcium influx, crossing the blood-brain barrier and inhibiting Aβ-protein aggregation

Type

journal article in Web of Science

Language

English

Original Abstract

Multifunctional nanoparticles could be the hallmark for the treatment of neurodegenerative diseases. Dissociation of protein aggregates causing neuronal damage and transfer of specific drugs which can downregulate neuronal excitotoxicity by inhibiting glutamatergic N-Methyl-D-Aspartate-receptors (NMDA) and then reducing calcium influx are among the main factors to consider for proper therapy. Here, we present a multiplatform based on nitrogen-doped graphene quantum dots (NGQDs) with such functionalities. The NGQDs were functionalized with Memantine, the clinically used drug, via covalent and non-covalent coupling, and we confirmed that the pharmaceutical activity was not altered. Apart from that, using xCELLigence technology and flow cytometric analysis of ABC transporter function, we uncovered that the ABC transporters of the blood-brain barrier (BBB) do not affect the ability of NGQD to cross BBB. Surprisingly, this study found that NGQDs have an inhibitory effect on NMDA receptors, thus supporting the action of Memantine. Moreover, NGQDs and their derivatives demonstrated the potential to dissociate beta-amyloid aggregates while possessing features suitable for bioimaging in various cell lines.

Keywords

Carbon dots; Memantine; Protein aggregation; Blood-brain barrier; Bioimaging

Authors

GOMEZ PEREZ, I.; KŘÍŽKOVÁ, P.; DOLEČKOVÁ, A.; CARDO, L.; WETZL, C.; PIZÚROVÁ, N.; PRATO, M.; MEDALOVÁ, J.; ZAJÍČKOVÁ, L.

Released

1. 2. 2024

Publisher

ELSEVIER

Location

AMSTERDAM

ISBN

2352-9407

Periodical

Applied Materials Today

Year of study

36

Number

102072

State

Kingdom of the Netherlands

Pages count

9

URL

https://www.sciencedirect.com/science/article/pii/S2352940724000180?via%3Dihub

BibTex

@article{BUT188954,
  author="Inmaculada Jennifer {Gomez Perez} and Petra {Křížková} and Anna {Dolečková} and Lucia {Cardo} and Cecília {Wetzl} and Naděžda {Pizúrová} and Maurizio {Prato} and Jiřina {Medalová} and Lenka {Zajíčková}",
  title="Multifunctional graphene quantum dots: A therapeutic strategy for neurodegenerative diseases by regulating calcium influx, crossing the blood-brain barrier and inhibiting Aβ-protein aggregation",
  journal="Applied Materials Today",
  year="2024",
  volume="36",
  number="102072",
  pages="9",
  doi="10.1016/j.apmt.2024.102072",
  issn="2352-9407",
  url="https://www.sciencedirect.com/science/article/pii/S2352940724000180?via%3Dihub"
}