Publication detail

Specific buffer effects on the formation of BSA protein corona around amino-functionalized mesoporous silica nanoparticles

MURA, M. CARUCCI, C. CADDEO, E. SOVOVÁ, Š. PILUDU, M. PEKAŘ, M. JACHIMSKA, B. PARSONS, D. E. SALIS, A.

Original Title

Specific buffer effects on the formation of BSA protein corona around amino-functionalized mesoporous silica nanoparticles

Type

journal article in Web of Science

Language

English

Original Abstract

The effect of buffer species on biomolecules and biomolecule-nanoparticle interactions is a phenomenon that has been either neglected, or not understood. Here, we study the formation of a BSA protein corona (PC) around amino-functionalized mesoporous silica nanoparticles (MSN-NH2) 2 ) in the presence of different buffers (Tris, BES, cacodylate, phosphate, and citrate) at the same pH (7.15) and different concentrations (10, 50, and 100 mM). We find that BSA adsorption is buffer specific, with the adsorbed amount of BSA being 4.4 times higher in the presence of 100 mM Tris (184 +/- 3 mg/g) than for 100 mM citrate (42 +/- 2 mg/g). That is a considerable difference that cannot be explained by conventional theories. The results become clearer if the interaction energies between BSA and MSN-NH2, 2 , considering the electric double layer (EEDL) EDL ) and the van der Waals (EvdW) vdW ) terms, are evaluated. The buffer specific PC derives from buffer specific zeta potentials that, for MSN-NH2, are positive with Tris and negative with citrate buffers. A reversed sign of zeta potentials can be obtained by considering polarizability-dependent dispersion forces acting together with electrostatics to give the buffer specific outcome. These results are relevant not only to our understanding of the formation of the PC but may also apply to other bio- and nanosystems in biological media.

Keywords

buffer; protein corona; silica nanoparticles

Authors

MURA, M.; CARUCCI, C.; CADDEO, E.; SOVOVÁ, Š.; PILUDU, M.; PEKAŘ, M.; JACHIMSKA, B.; PARSONS, D. E.; SALIS, A.

Released

5. 8. 2024

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE

Location

SAN DIEGO

ISBN

0021-9797

Periodical

Journal of Colloid and Interface Science

Year of study

677

Number

January

State

United States of America

Pages from

540

Pages to

547

Pages count

8

URL

https://www.sciencedirect.com/science/article/pii/S0021979724017727

BibTex

@article{BUT189337,
  author="MURA, M. and CARUCCI, C. and CADDEO, E. and SOVOVÁ, Š. and PILUDU, M. and PEKAŘ, M. and JACHIMSKA, B. and PARSONS, D. E. and SALIS, A.",
  title="Specific buffer effects on the formation of BSA protein corona around amino-functionalized mesoporous silica nanoparticles",
  journal="Journal of Colloid and Interface Science",
  year="2024",
  volume="677",
  number="January",
  pages="540--547",
  doi="10.1016/j.jcis.2024.07.258",
  issn="0021-9797",
  url="https://www.sciencedirect.com/science/article/pii/S0021979724017727"
}