Publication detail

Phage therapy combined with Gum Karaya injectable hydrogels for treatment of methicillin-resistant Staphylococcus aureus deep wound infection in a porcine model

VACEK, L. KLEKNEROVÁ, DP. LIPOVY, B. HOLOUBEK, J. MATYSKOVÁ, D. CERNÁ, E. BRTNIKOVÁ, J. JEKLOVÁ, E. KOBZOVÁ, S. JANDA, L. LISKOVÁ, L. DIABELKO, D. BOTKA, T. PANTUCEK, R. RUZICKA, F. VOJTOVÁ, L

Original Title

Phage therapy combined with Gum Karaya injectable hydrogels for treatment of methicillin-resistant Staphylococcus aureus deep wound infection in a porcine model

Type

journal article in Web of Science

Language

English

Original Abstract

Skin and soft tissue infections (SSTIs) represent a significant healthcare challenge, particularly in the context of increasing antibiotic resistance. This study investigates the efficacy of a novel therapeutic approach combining bacteriophage (phage) therapy with a gum Karaya (GK)-based hydrogel delivery system in a porcine model of deep staphylococcal SSTIs. The study exploits the lytic activity and safety of the Staphylococcus phage 812K1/ 420 of the Kayvirus genus, which is active against methicillin-resistant Staphylococcus aureus (MRSA). The GK injectable hydrogels and hydrogel films, developed by our research group, serve as effective, non-toxic, and easyto-apply delivery systems, supporting moist wound healing and re-epithelialization. In the porcine model, the combined treatment showed a synergistic effect, leading to a significant reduction in bacterial load (2.5 log CFU/ gram of tissue) within one week. Local signs of inflammation were significantly reduced by day 8, with clear evidence of re-epithelialization and wound contraction. Importantly, no adverse effects of the GK-based delivery system were observed throughout the study. The results highlight the potential of this innovative therapeutic approach to effectively treat deep staphylococcal SSTIs, providing a promising avenue for further research and clinical application in the field of infections caused by antibiotic-resistant bacteria.

Keywords

Bacteriophage; Phage therapy; Gum Karaya; Hydrogel film; Injectable hydrogel; Staphylococcus aureus; MRSA

Authors

VACEK, L.; KLEKNEROVÁ, DP.; LIPOVY, B.; HOLOUBEK, J.; MATYSKOVÁ, D.; CERNÁ, E.; BRTNIKOVÁ, J.; JEKLOVÁ, E.; KOBZOVÁ, S.; JANDA, L. ; LISKOVÁ, L.; DIABELKO, D.; BOTKA, T.; PANTUCEK, R.; RUZICKA, F.; VOJTOVÁ, L

Released

14. 6. 2024

Publisher

ELSEVIER

Location

AMSTERDAM

ISBN

1873-3476

Periodical

INTERNATIONAL JOURNAL OF PHARMACEUTICS

Year of study

660

Number

124348

State

Kingdom of the Netherlands

Pages count

11

URL

https://www.sciencedirect.com/science/article/pii/S0378517324005829?via%3Dihub

BibTex

@article{BUT189784,
  author="VACEK, L. and KLEKNEROVÁ, DP. and LIPOVY, B. and HOLOUBEK, J. and MATYSKOVÁ, D. and CERNÁ, E. and BRTNIKOVÁ, J. and JEKLOVÁ, E. and KOBZOVÁ, S. and JANDA, L. and LISKOVÁ, L. and DIABELKO, D. and BOTKA, T. and PANTUCEK, R. and RUZICKA, F. and VOJTOVÁ, L",
  title="Phage therapy combined with Gum Karaya injectable hydrogels for treatment of methicillin-resistant Staphylococcus aureus deep wound infection in a porcine model",
  journal="INTERNATIONAL JOURNAL OF PHARMACEUTICS",
  year="2024",
  volume="660",
  number="124348",
  pages="11",
  doi="10.1016/j.ijpharm.2024.124348",
  issn="1873-3476",
  url="https://www.sciencedirect.com/science/article/pii/S0378517324005829?via%3Dihub"
}