Publication detail

Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment

KROUPOVÁ, Z. SLANINOVÁ, E. MRÁZOVÁ, K. KRZYŽÁNEK, V. HRUBANOVÁ, K. FRITZ, I. OBRUČA, S.

Original Title

Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment

Type

journal article in Web of Science

Language

English

Original Abstract

Cyanobacteria are prokaryotic organisms characterised by their complex structures and a wide range of pigments. With their ability to fix CO2, cyanobacteria are interesting for white biotechnology as cell factories to produce various high-value metabolites such as polyhydroxyalkanoates, pigments, or proteins. White biotechnology is the industrial production and processing of chemicals, materials, and energy using microorganisms. It is known that exposing cyanobacteria to low levels of stressors can induce the production of secondary metabolites. Understanding of this phenomenon, known as hormesis, can involve the strategic application of controlled stressors to enhance the production of specific metabolites. Consequently, precise measurement of cyanobacterial viability becomes crucial for process control. However, there is no established reliable and quick viability assay protocol for cyanobacteria since the task is challenging due to strong interferences of autofluorescence signals of intercellular pigments and fluorescent viability probes when flow cytometry is used. We performed the screening of selected fluorescent viability probes used frequently in bacteria viability assays. The results of our investigation demonstrated the efficacy and reliability of three widely utilised types of viability probes for the assessment of the viability of Synechocystis strains. The developed technique can be possibly utilised for the evaluation of the importance of polyhydroxyalkanoates for cyanobacterial cultures with respect to selected stressor-repeated freezing and thawing. The results indicated that the presence of polyhydroxyalkanoate granules in cyanobacterial cells could hypothetically contribute to the survival of repeated freezing and thawing.

Keywords

Biotechnology; Cyanobacteria; Flow cytometry; Fluorescent viability probes; Stress resistance; Viability assessment

Authors

KROUPOVÁ, Z.; SLANINOVÁ, E.; MRÁZOVÁ, K.; KRZYŽÁNEK, V.; HRUBANOVÁ, K.; FRITZ, I.; OBRUČA, S.

Released

6. 11. 2024

Publisher

SPRINGER

Location

DORDRECHT

ISBN

1874-9356

Periodical

FOLIA MICROBIOLOGICA

Year of study

70

Number

jaro

State

Czech Republic

Pages count

19

URL

https://link.springer.com/article/10.1007/s12223-024-01212-w#citeas

BibTex

@article{BUT193545,
  author="Zuzana {Kroupová} and Eva {Slaninová} and Kateřina {Mrázová} and Vladislav {Krzyžánek} and Kamila {Hrubanová} and Ines {Fritz} and Stanislav {Obruča}",
  title="Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment",
  journal="FOLIA MICROBIOLOGICA",
  year="2024",
  volume="70",
  number="jaro",
  pages="19",
  doi="10.1007/s12223-024-01212-w",
  issn="1874-9356",
  url="https://link.springer.com/article/10.1007/s12223-024-01212-w#citeas"
}