Publication detail

Two colorimetric LAMP systems for nucleic acid-based diagnostics

SUN, A. STEJSKALOVÁ, P. LIU, X. ŘEZNÍČEK, T. BRODSKÝ, J. GABLECH, I. ZÍTKA, O. NEUŽIL, P.

Original Title

Two colorimetric LAMP systems for nucleic acid-based diagnostics

Type

journal article in Web of Science

Language

English

Original Abstract

Background: This study introduces an advanced 8-well loop-mediated isothermal amplification (LAMP) system specifically designed for the automated colorimetric detection of SARS-CoV-2. Incorporating two distinct configurations having either three light-emitting diodes (LEDs) with varying emission wavelengths per well, paired with a photodiode detector, or utilizing white LED illumination with a red, green, and blue (RGB) sensor. The colorimetric LAMP aims to provide a more accessible and rapid diagnostic tool than traditional fluorescence methods due to the system's simplicity. Results: We designed, assembled, and compared two colorimetric home-assembled LAMP systems, the first one based on three LEDs, each with a different color with a photodiode, and the second one having RGB and a white LED, with traditional fluorescence-based LAMP method performed on a commercial qPCR instrument. Results demonstrated that the colorimetric RT-LAMP assays achieved critical threshold time (CT), closely matching the CT value of fluorescence-based detection accomplished by the qPCR instrument. We performed the fundamental experiment employing an identical RNA copy number of 1,570copies center dot mu L-1, getting the CT value of (16.70 f 0.43) min (mean f standard deviation from 23 measurements). Then, we also performed different RNA numbers of copies between the highest and lowest RNA contents of ti 157,000 copies center dot mu L-1 and ti 1570 copies center dot mu L- 1, respectively, getting CT values from (13.30 f 0.04) min to (13.75 f 0.30) min and (17.04 f 0.02) min to (17.26 f 0.02) min, all (mean f standard deviation from three measurements). The colorimetric systems demonstrated rapid response and precision across varied viral loads while keeping the system simple due to the colorimetric detection method. Significance and novelty: The LAMP system's rapid and precise detection capabilities underscore its potential as an effective tool for point-of-need diagnostics. It is crucial for timely responses in ongoing and future pandemic scenarios. This system enhances testing accessibility and provides a robust platform for potential adaptation to other pathogenic threats, making it a valuable asset in global health diagnostics.

Keywords

Loop-mediated isothermal amplification (LAMP); Colorimetric detection; SARS-CoV-2 diagnostics; Rapid testing technologies; Point-of-care diagnostics; Biosensors and actuators

Authors

SUN, A.; STEJSKALOVÁ, P.; LIU, X.; ŘEZNÍČEK, T.; BRODSKÝ, J.; GABLECH, I.; ZÍTKA, O.; NEUŽIL, P.

Released

8. 4. 2025

Publisher

ELSEVIER

Location

NETHERLANDS

ISBN

0003-2670

Periodical

ANALYTICA CHIMICA ACTA

Year of study

1346

Number

4

State

Kingdom of the Netherlands

Pages from

1

Pages to

11

Pages count

11

URL

https://www.sciencedirect.com/science/article/pii/S0003267025001461?via%3Dihub

BibTex

@article{BUT197137,
  author="SUN, A. and STEJSKALOVÁ, P. and LIU, X. and ŘEZNÍČEK, T. and BRODSKÝ, J. and GABLECH, I. and ZÍTKA, O. and NEUŽIL, P.",
  title="Two colorimetric LAMP systems for nucleic acid-based diagnostics",
  journal="ANALYTICA CHIMICA ACTA",
  year="2025",
  volume="1346",
  number="4",
  pages="11",
  doi="10.1016/j.aca.2025.343752",
  issn="0003-2670",
  url="https://www.sciencedirect.com/science/article/pii/S0003267025001461?via%3Dihub"
}