Publication detail

Comparison of Two Procedures of Loading with Voltage-Sensitive Dye Di-4-ANEPPS in Rabbit Isolated Heart

NOVÁKOVÁ, M. NOGOVÁ, K. BARDOŇOVÁ, J. PROVAZNÍK, I.

Original Title

Comparison of Two Procedures of Loading with Voltage-Sensitive Dye Di-4-ANEPPS in Rabbit Isolated Heart

Type

conference paper

Language

English

Original Abstract

Recording of monophasic action potentials (MAPs) by optical method using voltage sensitive dyes has been established as possibility to measure membrane potential changes in certain models. We studied two procedures of isolated rabbit hearts loading with di-4-ANEPPS - "slow" method (perfusion with low concentration of dye for longer time) or "fast" method (single microinjection of highly concentrated bolus of the dye in the aorta). Signal-to-noise ratio was evaluated in each minute of continuous exposure to excitatory light and persistence of dye in the tissue was estimated. We conclude that both methods enable us to record MAPs, but during long exposure to light the persistence of the dye in the tissue is insufficient in "fast" method. Although the "slow" loading affects electrophysiological properties of the heart, in case of rabbit heart it is negligible since its relative resistance to the side-effects of di-4-ANEPPS.

Keywords

Recording, Voltage-Sensitive Dye, Action Potentials

Authors

NOVÁKOVÁ, M.; NOGOVÁ, K.; BARDOŇOVÁ, J.; PROVAZNÍK, I.

RIV year

2008

Released

1. 12. 2008

Publisher

IEEE

Location

Bologna, Italy

ISBN

978-1-4244-3706-1

Book

Computers in Cardiology

ISBN

0276-6574

Periodical

Computers in Cardiology

State

United States of America

Pages from

1

Pages to

4

Pages count

4

BibTex

@inproceedings{BUT27047,
  author="Marie {Nováková} and Kateřina {Nogová} and Jana {Kolářová} and Valentine {Provazník}",
  title="Comparison of Two Procedures of Loading with Voltage-Sensitive Dye Di-4-ANEPPS in Rabbit Isolated Heart",
  booktitle="Computers in Cardiology",
  year="2008",
  journal="Computers in Cardiology",
  pages="1--4",
  publisher="IEEE",
  address="Bologna, Italy",
  isbn="978-1-4244-3706-1",
  issn="0276-6574"
}