Publication detail

Specification and simplified analyzing data from MS/MS spectrometry

VALLA, M. PLCHÚT, M. PROVAZNÍK, I.

Original Title

Specification and simplified analyzing data from MS/MS spectrometry

Type

conference paper

Language

English

Original Abstract

The goal of this work was to design a suitable method for unifying score values from various identification tools of proteins identification in tandem mass spectrometry into a single score value. Output data from the mass spectrometric method were processed by two independent search tools Mascot and X!Tandem. Those were selected especially for their wide applications in proteomic laboratories. Results of both methods were evaluated through a newly designed function and unified to a single valued score that clearly identifies found proteins. The newly designed function of producing of scored values was termed Metascore and implemented into MATLAB computational environment. Given results were successfully tested on selected real data available in public databases of proteomic sequences

Keywords

Score, Mascot, X!Tandem, Metascore, MS/MS, peptide mass fingerprint, tandem mass spectrometry, MS/MS, spectrometry

Authors

VALLA, M.; PLCHÚT, M.; PROVAZNÍK, I.

RIV year

2009

Released

7. 9. 2009

Publisher

IFMBE

ISBN

978-3-642-03881-5

Book

Proc. World Congress of Medical Physics and Biomedical Engineering

Pages from

1510

Pages to

1512

Pages count

3

URL

http://conference.vde.com/world-congress-2009/Pages/Home.aspx

BibTex

@inproceedings{BUT31402,
  author="Martin {Valla} and Martin {Plchút} and Valentine {Provazník}",
  title="Specification and simplified analyzing data from MS/MS spectrometry",
  booktitle="Proc. World Congress of Medical Physics and Biomedical Engineering",
  year="2009",
  pages="1510--1512",
  publisher="IFMBE",
  isbn="978-3-642-03881-5",
  url="http://conference.vde.com/world-congress-2009/Pages/Home.aspx"
}